脂肪酶催化的研究进展  

Advance in Lipase Catalysis

作　　者：刘书来[1] 

机构地区：[1]郑州工程学院,河南郑州450052

出　　处：《化工科技市场》2003年第4期16-20,共5页Chemical Technology Market

摘　　要：本文简单介绍了脂肪酶的立体结构和催化机理,综述了非水相的有机溶剂体系中脂肪酶催化的特点与性质,并对微水的无溶剂体系、微乳液体系和超临界体系进行了简单的论述。定向进化技术有助于改善酶的催化特点和应用范围,酶交联晶体的制备和生物印迹方法的采用对提高脂肪酶的活性、热稳定性和选择性也有较大的促进作用。The structure and catalytic mechanism of lipase was presented briefly. The characteristics of lipase in the non - aqueous sys-tem( including solvent - free system, reverse - phase micelle and supercritical fluid) were discussed. Directed evolution will improve the catalytic properties and broaden the range of its application. Cross - linked enzyme crystal and imprinting of lipase will be helpful for its catalytic activity, thermal stability and stereoselectivity.

关 键 词：脂肪酶 催化 研究进展 立体结构 催化机理 非水相 定向进化 交联酶晶体 生物印迹 

分 类 号：O643.36[理学—物理化学] Q814[理学—化学]