产低温纤维素酶海洋嗜冷菌的筛选及研究  

SCREENING AND CHARACTERIZATION OF THE COLD-ADAPTIVE CELLULASE-PRODUCING BACTERIA

作　　者：王玢[1] 汪天虹[1] 张刚[1] 刘世利[1] 肖天[2] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100 [2]中国科学院海洋研究所,青岛266071

出　　处：《海洋科学》2003年第5期42-45,共4页Marine Sciences

摘　　要：从黄海的深海海底泥样中筛选出一株产纤维素酶的海洋细菌,初步鉴定为革兰氏阴性杆菌。该菌既能产生装甲基纤维素酶,又能降解微晶纤维素,且有淀粉酶活。对该菌生长特征及所产纤维素酶的性质进行了初步研究。此菌最适生长温度为20℃,最高生长温度为40℃,在0℃也能生长,是典型的嗜冷菌。该菌所产纤维素酶最适反应温度为35℃,10℃仍有较高酶活,最适pH值为6.0,属酸性酶。One strain of cold-adaptive cellulase-producing bacteria which can degrade carboxymethyl cellulase (CMC) and Avicel was isolated frcm the submarine of the Yellow Sea and the East China Sea. The strain dencminated MBl with high cellulase production was further studied on the growth characteristics and enzyme properties. The optimum and highest growth temperatures for MB1 are 20℃ and 40 ℃ , respectively. The optimum temperature for the CMCase is 35℃ and the optimum pH is 6.0. But even under 10 ℃, the enzyme still shows some activities .It is expected that the cold-adapted cellulase could be used in textile, paper making , food industry ,fodder industry and so on.

关 键 词：温纤维素酶 海洋嗜冷菌 筛选 酸性酶 生长温度 

分 类 号：Q936[生物学—微生物学] Q93－3