饲用复合酶产生菌好食脉孢霉发酵条件研究  

STUDY ON FERMENT CONDITIONS OF FEED COMPLEX ENZYMES PRODUCED BY A NEUROSPORA SP.

作　　者：吴慧清[1] 吴清平[1] 郭楚盛[1] 李剑英[1] 刘淑欢[1] 

机构地区：[1]广东省微生物研究所,广州510070

出　　处：《微生物学通报》2003年第3期33-37,共5页Microbiology China

摘　　要：利用好食脉孢霉发酵蔗渣、麸皮、木薯渣等工农业有机废料 ,生产可用于配制饲用复合酶制剂的酶制品。合适的培养基配方为每千克固体料中含蔗渣 10 0 g、麸皮 6 0 0g、木薯渣 30 0 g ,并添加 2 0g复合氮源和适量的磷酸盐 ,培养基含水量为固体料干重的两倍 ;接种后先在 34℃以下培养 2d ,然后在 37℃发酵 2～ 3d ,发酵过程中无光照、空气供应充分、培养基中含水量损失不能太大。发酵结束后固体曲中纤维素酶活力 (CMC +C1) 1,75 9mg/g/h ,糖化酶活力 3,110mg/ g/h ,蛋白酶活力 2 97mg/ g/h。另一方面 ,低温、光照、供气充分。A Neurospora sp was used to ferment organic waste materials such as sugarcane residue powder (bagasse), bran and cassava residue The feed complex enzymes including cellulase, saccharifying enzyme,neutral proteinase and other growth factors were produced The experimental results showed that suitable medium contained sugarcane residue powder 100 g ?bran 600 g, cassava residue 300 g, mixed nitrogen sucrose 20 g per kilogram solid materials and some content of phosphate, the water in medium was twice as weight as the solid materials;the suitable fermentation conditions were that the strain was cultured first for 2 days under 34℃ and then fermented for 2～3 days at 37℃, no light,keeping the watering medium and suppling enough oxygen during fermentation The cellulase (CMC+C1) was 1759mg/g/h, saccharifying enzyme to 3110 mg/g/h, neutral proteinase 297mg/g /h in ferment product On the other hand, light,enough air,lower temperature and less water content in medium were benefit to producing spores

关 键 词：好食脉孢霉 有机废料 饲用复合酶 孢子形成 

分 类 号：TQ920[轻工技术与工程—发酵工程]