酵母醇脱氢酶的分离纯化  

Separation and Purification of Alcohol Dehydrogenase from Yeast

作　　者：柳畅先[1] 吴士筠[1] 李志勇[1] 

机构地区：[1]中南民族大学化学与生命科学学院

出　　处：《中南民族大学学报（自然科学版）》2003年第2期11-12,共2页Journal of South-Central University for Nationalities：Natural Science Edition

摘　　要：报道了酵母醇脱氢酶的分离纯化 .使用硫酸铵分级沉淀 ,Sephadex G- 1 0 0凝胶层析技术 ,从啤酒酵母提取液中分离出醇脱氢酶 .收得率为 1 0 .9% ,纯化倍数达 5 3.The procedure for the separation and purification of alcohol dehydrogenase from yeast was studied.The crude extract was treated by saturated ammonium sulfate precipitation,then purified by Sephadex G 100 column chromatography.The yield of alcohol dehydrogenase was 10.9%,and purification flod reached 53.2.

关 键 词：醇脱氢酶 分离纯化 酵母 硫酸铵分级沉淀 凝胶层析 

分 类 号：TQ925[轻工技术与工程—发酵工程]