反相高效液相色谱法测定发酵液中的纳他霉素  

DETERMINATION OF NATAMYCIN IN FERMENTATION BROTH BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：陈冠群[1] 杨东靖[1] 杜连祥[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院,天津300222

出　　处：《天津轻工业学院学报》2003年第1期9-11,共3页Journal of Tianjin University of Light Industry

基　　金：天津市自然科学基金项目(013609511)

摘　　要：采用反相高效液相色谱法测定了发酵液中的纳他霉素。在Phenomenexprodigy5μODS3100A(250mm×4.60mmi.d.,5μm)色谱柱上,以甲醇 水 磷酸(体积比85∶15∶0 15)为流动相,体积流速为1mL/min,紫外检测波长为303nm。实验结果表明,该方法的相对标准偏差为0 24%(n=5),回收率为99%以上。A method for determining natamycin in fermentation broth by RPHPLC is described.The operating conditions were Phenomenex prodigy 5μ ODS3 100A(250 mm×4.60 mm i.d.,5 μm)at room temperature,methanolwaterphosphoric acid(85∶15∶0.15,V/V)as mobile phase with a flow rate of 1.0 mL/min and UV detection at 303 nm.The retention time of natamycin was 1.972 min.This method is simple,rapid and accurate.The results will not be affected by other components in the broth.The relative standard deviation was 0.24%(n=5), and the recovery was over 99%.

关 键 词：反相高效液相色谱法 测定 发酵液 纳他霉素 色谱柱 

分 类 号：TQ927[轻工技术与工程—发酵工程]