基于代谢流导向与分析的L-异亮氨酸发酵条件优化  

Condition Optimum of L-isoleucine Fermentation Based on Metabolic Flux Direction and Analysis

作　　者：宋文军[1] 陈宁[1] 魏春[1] 张克旭[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院,天津300222

出　　处：《天津轻工业学院学报》2003年第2期15-19,共5页Journal of Tianjin University of Light Industry

摘　　要：基于代谢流导向与分析,对L 异亮氨酸种子培养基中碳氮源组合、豆饼水解液用量和种子pH控制条件进行了研究,得到了优化的种子培养条件;采用均匀设计法考察了发酵培养基中几种主要成分对发酵的影响,得到葡萄糖、(NH4)2SO4、VH、VB、VB1的最适用量,分析了这4种组分对增大L 异亮氨酸合成代谢流的作用,并对最适供氧量进行了研究,得到最佳发酵条件,在此条件下产L 异亮氨酸19.1g/L。Based on indirection and analysis of metabolic flux, we studied the concentration of carbon and nitrogen souce ,soy bean hydrolyzate and pH control condition. Then we got the optimum seed cultue condition; Uniform design was employed in our paper to test the influence of several major components in fermentation medium to isoleucine fermentation .We obtained the optimum concentrations of glucose, (NH4)2SO4,VH and VB1 ,then we analysed the influence on accerelating the metabolic flux to above four components.The oxygen supplying conditions was studied in shakeflask.Under the optimum conditions,the strain could produce isoleucine 19.1 g/L.

关 键 词：代谢流导向 L—异亮氨酸 发酵条件 优化 均匀设计法 发酵培养基 种子培养基 

分 类 号：TQ922.9[轻工技术与工程—发酵工程]