氮源与无机盐对高浓度酒精发酵的影响  

Study on the effects of nitrogen sources and inorganic salts on ethanol fermentation

作　　者：吕欣[1] 段作营[1] 毛忠贵[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214036

出　　处：《西北农林科技大学学报（自然科学版）》2003年第4期159-162,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：科技部科技型中小企业技术创新基金无偿资助项目 ( 0 1C2 62 13 2 0 0 3 2 5 )

摘　　要：在完全合成培养基条件下 ,就尿素、硫酸铵 2种氮源和硫酸镁、磷酸二氢钾、氯化钙 3种无机盐对 1株产高浓度酒精的酿酒酵母的间歇发酵影响进行了初步研究。结果表明 ,与尿素相比硫酸铵是较好的无机氮源 ,发酵终了酒精浓度可达 119.9g/L;并初步得到该株酵母发酵所需各无机盐的临界浓度分别为 :硫酸镁 5 g/L,磷酸二氢钾 5 g/L(或 <5 g/L) ,氯化钙 5 g/L。The effects of nitrogen sources and inorganic salt on high ethanol fermentation in synthetic medium were studied in this paper.Ammonium sulfate was the better nitrogen source between urea and ammonium sulfate,the ultimate ethanol concentration was 119.9 g/L when we used ammonium sulfate as nitrogen source.Moreover,the effects of calcium chloride,magnesium sulfate and potassium biphosphate on high ethanol fermentation were analyzed,the critical concentrations of these inorganic salt the Saccharomyces cerevisiae needed were gained.

关 键 词：氮源 无机盐 浓度 酒精发酵 影响因素 培养基 临界浓度 

分 类 号：TS261.43[轻工技术与工程—发酵工程]