对苯二甲酸降解酵母及其活性  

Yeast degrading terephthalic acid activities.

作　　者：陈鹏[1] 肖琳[1] 吴剑[1] 刘慧[1] 杨柳燕[1] 朱建中[2] 

机构地区：[1]南京大学环境学院,污染控制与资源化研究国家重点实验室,江苏南京210093 [2]南京扬子石化公司,江苏南京210048

出　　处：《中国环境科学》2003年第4期412-416,共5页China Environmental Science

基　　金：南京市科技发展项目(20022035)

摘　　要：通过半连续驯化,从对苯二甲酸活性污泥中分离到一株能降解对苯二甲酸(TA)的假丝酵母(Candida),命名为CP01.经两次紫外诱变后,获得了能以TA为唯一碳源的高效酵母菌CP02,诱变前后酵母全细胞蛋白聚丙烯酰胺凝胶电泳(SDS-PAGE)条带有明显差异. CP02在30℃, pH5.5～8,振荡速率150r/min的培养条件下,72h内对1400mg/L的TA降解率可达80%以上.A Candida strain named CP01 which could degrade terephthalic acid(TA)was isolated by semi-continuous taming technique from TA activated sludge. A high effective yeast CP02 utilizing TA as sole carbon source was obtained with two times of UV mutagenesis; and the SDS-PAGE of protein in yeast was markedly different before and after the mutagenesis. The degradation rate of 1400 mg/L TA in 72h could exceed 80% under the cultivation conditions of 30℃, pH5.5~8 and shaking speed 150r/min.

关 键 词：对苯二甲酸 酵母菌 紫外诱变 聚丙烯酰胺凝胶电泳 降解 

分 类 号：X172[环境科学与工程—环境科学]