蛹虫草液体培养条件的优化及生长动力学考察  

The Determination of Optimal Culture Conditions and Biodynamics for Cordyceps militaris

作　　者：汪宇[1] 于荣敏[2] 佟志清[1] 李媛[1] 宾文[3] 

机构地区：[1]中国人民解放军第四六三医院药剂科,辽宁沈阳110042 [2]暨南大学药学院,广东广州510632 [3]沈阳药科大学制药工程学院,辽宁沈阳110016

出　　处：《中国野生植物资源》2003年第4期56-60,共5页Chinese Wild Plant Resources

摘　　要：为了优化蛹虫草菌的液体培养条件 ,对液体培养菌丝的生长情况进行了研究。采用摇瓶培养的方法 ,以发酵得率为指标确定培养条件。结果表明 :优化培养基成分确定为蛋白胨 1.5 % ,葡萄糖 2 % ,KH2 PO4 0 .15 % ,Mg SO40 .0 5 %、VB1 5 m g/ L .2 ,4 - D 2 mg/ L .该培养基下发酵得率达 1.4～ 2 .2 g/ 10 0 m L ;考察了蛹虫草菌液体培养的外部条件 ,确定为 :装料系数为 30 % ,培养基 p H为 5 .5 ,接种量为 0 .0 3g,摇床振荡频率为 12 0 r· m in- 1 ;利用优化培养基得到了生长代谢曲线。在相同条件下 ,优化培养基比原来培养基的发酵得率提高了 4 .5 % ,研究初步得到蛹虫草菌液体培养条件和生长动力学 。To study the optimal conditions and biodynamics for liquid culture of Cordyceps militaris were studied. The culture conditions were established in shake flask using fermentation rates as marker. The optimal medium were peptone 1.5%,glucose 2%,potassium biphosphate 0.15% ,magnesium sulfate 0.05% ,vitamin B 1 5mg/L,2,4 D 2mg/L, respectively. Under the optimal medium, the fermentation rates were 1.4-2.2 g/100ml. As the other conditions, charge coefficient? initial pH? agitation frequency and inoculate weight were found to be 30%?5.5?120 r·min -1 and 0.03g, respectively. Meanwhile, growth curves were confirmed. Under the same conditions, the fermentation rate with optimal medium was raised 4.5%,which can have a certain impact on the industrial scale.

关 键 词：蛹虫草 液体培养 生长动力学 培养条件 药用真菌 发酵 

分 类 号：TQ92[轻工技术与工程—发酵工程] R282.7[医药卫生—中药学]