非小细胞肺癌患者血清DNA中p53基因杂合性丢失的研究  被引量:2

Loss of heterozygosity at p53 in serum DNA from non-small cell lung cancer patients

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作  者:李红[1] 包国良[2] 许凯黎[1] 

机构地区:[1]上海交通大学肿瘤研究所 [2]上海市胸科医院肺癌研究所

出  处:《肿瘤》2003年第4期318-320,共3页Tumor

基  金:上海市医学发展基金重点研究课题项目 (0 14 1190 13 )

摘  要:目的 通过对非小细胞肺癌 (non—smallcelllungcancer,NSCLC)患者血清DNAp5 3等位基因杂合性丢失 (LossofHet erozygosity ,LOH)的研究 ,拟探讨肺癌患者来源的循环DNA与原发性癌组织DNA基因变异相关性及其临床意义。方法 采用PCR并结合二核苷酸 (CA)n重复序列多态性的方法 ,分别对 4 3例NSCLC、4例良性肺疾患及 2 0例正常人血清DNA中 p5 3基因的 2个微卫星标志 (TP5 3、D17S786 )进行杂合性丢失检测。结果  4 3例NSCLC血清DNA标本中 2 4例患者 (5 5 .81% )至少存在 1个位点发生LOH以及 8例患者 (18.6 0 % )可出现 2个位点双丢失现象 ,而 4例良性肺疾患和 2 0例正常人血清DNA均未观察到LOH。NSCLC患者血清DNA中p5 3基因杂合性丢失频率与TNM分期和组织学分类无相关性。结论 鉴于血清DNAp5 3LOH在NSCLCⅠ期患者出现较高的丢失频率 ,故检测NSCLC患者血清DNAp5 3等位基因的LOH有望与其他肿瘤标志物联合检测作为NSCLC早期诊断的指标之一。Objective Aim ofthe study is to investigatethefrequency of LOH(Loss of Heterozygosity)atp53 inthe serum DNA from non-small eellIung cancer and its clinical application.Methods There were 43 serum DNA samples from non-small eelIlung cancer,4samples from benignlung diseases and20 samplesfromnormal persons collectedfmmShanghaiChestHospital.AllDNA samplesisolated frwn sera by Genomic DNA micro kit were examined for LOH by using PCR with two polymorphic microsatellite markers.p53(TP53、D17S786).Results It was found that in24 out of 43(55.81%)NSCLC sertllTl samplestheLOHoccurredin atleast one oftwoloci,while 8 of them(16.80%)exhibited LOH at two loci.No LOHWaS detected in4 cases with benign lung diseases and in20 normal persons.However.the results showed that the frequency of LOH at 17p is not related to TNM staging and pathological classification of NSCLC.Conclusion Sincep53 LOH could be detectable in 47.37%NSCLC patients with pathological stage I,the detection of LOH in serumDNAmaybe used aS amolecularmarker combinedwith other bicxnarkersfor earlytumor diagnosis.

关 键 词:非小细胞肺癌 P53基因 杂合性丢失 循环DNA 基因变异 

分 类 号:R734.2[医药卫生—肿瘤]

 

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