泥土芽孢杆菌YMTC1049菌株高温蛋白酶产酶条件的优化  

Optizing cultivation condition of thermophilic protease from Geobacillus sp.YMTC1049 strain

作　　者：祝伟[1] 彭谦[1] 李勇 郭春雷[1] 杨红亚[1] 

机构地区：[1]云南大学云南省微生物研究所教育部微生物资源开放研究重点实验室,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2003年第5期463-468,共6页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(39960003);云南省工业微生物发酵工程重点实验室开放基金资助项目.

摘　　要：报道高温蛋白酶产生菌YMTC1049(Geobacillussp.)产酶条件的优化过程.在基础培养基中分别添加葡萄糖、麦芽糖、酵母提取物、蛋白胨、胰蛋白胨、酪蛋白、聚胨等7种营养成分,获得对酶活影响较大的碳、氮源.用多因素正交试验设计考察了pH、酪蛋白、葡萄糖和温度对酶活的影响水平,菌株在优化发酵条件下培养24h时,上清液蛋白酶活力达312U/(mL·min).The factors affect the production of thermophilic protease from Geobacillus sp.YMTC1049 strain were reported.Among the nutrilits which include glucose,maltose,yeast extract,casein,tryptone,polypeptone,and peptone,YMTC1049 strain digests glucose and casein easier than others.By orthogonal test,influence of various factors on enzyme production of YMTC1049 strain was investigated,the suitable condition of YMTC1049 strain fermention was obtained.312 units of thermophilic protease activity in 1 mL of fermented culture could be achieved under the suitable condition.

关 键 词：泥土芽孢杆菌 YMTCl049菌株 高温蛋白酶 产酶条件 酶活力 热稳定性 发酵条件 

分 类 号：TQ925.2[轻工技术与工程—发酵工程] TQ920.1