血清中二噁英类物质的同位素稀释-全二维气相色谱-串联质谱法研究  

Investigation of polychlorinated dibenzo-p-dioxins in serum by isotope dilution-comprehensive two dimensional gas chromatography-tandem mass spectrometry

作　　者：尹帅星 吕冰[2] 张磊[2] 李敬光[2] 吴永宁[1,2] 

机构地区：[1]武汉轻工大学食品科学与工程学院,农产品加工湖北省协同创新中心,武汉430000 [2]国家食品安全风险评估中心卫生部食品安全风险评估重点实验室,北京100021

出　　处：《食品安全质量检测学报》2015年第3期992-999,共8页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(21361140359)~~

摘　　要：目的建立高灵敏度和高选择性的全二维气相色谱-串联质谱法(GC×GC-MS/MS)测定17种多氯代二苯并二噁英和多氯代二苯并呋喃(PCDD/Fs)同系物的痕量分析方法。方法将牛血清样品经过加速溶剂萃取,全自动净化仪净化后,通过配备DB-5MS和BPX-50柱全二维气相色谱-串联质谱进行样品分析。结果 17种PCDD/Fs同系物的标准曲线在范围内显示良好线性(R2】0.99)。方法的检出限为0.1~0.8 pg/g。结论本方法满足血清样品中PCDD/Fs的痕量分析需求。Objective A method based on comprehensive two dimensional gas chromatography-tandem mass spectrometry (GC×GC-MS/MS) was developed for the determination of polychlorinated dibenzo-p-dioxins (PCDD/Fs). Methods Bovine serum were extracted by accelerated solvent extraction and purified by fluid management system. Then the target congeners were analysed by comprehensive two dimensional gas chromatography-tandem mass spectrometry with DB-5MSand BPX-50. Results The calibration curves of 17th target compounds showed a good linearity (R2>0.99), the limits of detection of the method were 0.1~0.8 pg/g. Conclusion With high selectivity and sensitivity, the method can apply to analyze the PCDD/Fs in bovine serum.

关 键 词：全二维气相色谱-串联质谱法 同位素稀释法 多氯代二苯并二噁英和多氯代二苯并呋喃 

分 类 号：O657.63[理学—分析化学] R446.11[理学—化学]