免疫胶体金快速检测水产品中孔雀石绿残留的质量分析和评价  

Evaluation of qualities of colloidal gold rapid detection kits for malachite green in seafood

作　　者：刘欢[1] 郭萌萌[2] 李晋成[1] 吴立冬[1] 许玉艳[1] 李强[1] 马兵[1] 

机构地区：[1]中国水产科学研究院质量与标准研究中心,北京100141 [2]黄海水产研究所国家水产品质量监督检验中心,青岛266071

出　　处：《食品安全质量检测学报》2015年第5期1675-1682,共8页Journal of Food Safety and Quality

基　　金：国家自然科学基金资助项目(20907051);中央级公益性科研院所基本科研业务费专项资金资助项目(2013C006)。~~

摘　　要：目的为推动免疫胶体金类现场快速检测技术在水产品药物残留监管领域中的应用。方法本研究采用一套"盲样式"免疫胶体金类快速检测产品质量评价方法,对10种市售的用于检测水产品中孔雀石绿残留的国产胶体金快速检测产品的假阳性率、假阴性率、检出率及稳定性等指标进行验证。结果结果显示,有3种产品能100%准确检测出84个鱼糜样品中是否残留孔雀石绿,且2个不同批次产品的检测结果一致;另外7种产品检出假阳性或假阴性样品,且2个不同批次间产品的稳定性有差异。结论将此类产品应用于水产品质量安全监管之前,有必要对其最低检出限、假阳性率、假阴性率、稳定性等参数进行验证,以确保检测结果的科学性和公正性。Objective To promote the application of colloidal gold rapid detection kits in sea food residual regulation. Methods Ten kinds of rapid detection products which were used to detect the residue of malachite green were tested and evaluated by a kind of blind sample testing method, which included false positive rate (FPR), false negative rate (FNR), and the lowest detectable limit (LDL). Results The results showed that only 3 kinds of product could detect the malachite green residues in 84 fish samples exactly, and there was no difference between the 2 results using different batch of products. The other products detected the false positive/ negative samples, there were different stability in FPR/ FNR of these products when using 2 batches of products. Conculusion The results suggest that it is necessary to evaluate the LDL, FPR and FNR to ensure the impartiality of the test results before application.

关 键 词：孔雀石绿 免疫胶体金类快速检测产品 分析评价 假阳性率 假阴性率 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]