离子沉淀法分离八月瓜果皮中的总多酚  

Separation of Total Polyphenols from Akebia trifoliate Peel by Calcium Precipitation Method

作　　者：欧阳玉祝[1] 李雪峰[1] 姚懿桓[1] 

机构地区：[1]吉首大学食品科学研究所,湖南吉首416000

出　　处：《食品科学》2014年第16期76-79,共4页Food Science

基　　金：吉首大学植物资源保护与利用湖南省高校重点实验室开放基金项目(13jdzb094);湖南湘西自治州科技计划项目(州财企函[2013]7号)

摘　　要：以八月瓜果皮干粉为原料分离总多酚,在单因素试验基础上,采用响应面法优化钙沉淀分离总多酚的工艺条件,考察沉淀剂用量、沉淀温度和时间对总多酚提取率的影响。结果表明,钙沉淀分离总多酚的最佳工艺条件为:在50 mL提取液中,添加90 mL饱和石灰水,70℃沉淀38 min。总多酚提取率为5.1037%。试验模型的预测值与组合实验结果吻合,分离产品总多酚纯度为84.4174%。A calcium precipitation method to separate total polyphenols from a 70% ethanol extract of dried powdered Akebia trifoliate peel was proposed and optimized by response surface methodology. The yield of total polyphenols was investigated as a function of precipitator dosage, precipitation temperature and time. The results showed that the optimum precipitation conditions that provided maximum total polyphenol yield of 5.1037% were obtained when 50 mL of the extract was precipitated by adding 90 mL of clear saturated limewater at 70 ℃ for 38 min. There was a good agreement between the predicted and experimental data for total polyphenol yield. The separated precipitate contained 84.4174% total polyphenols.

关 键 词：八月瓜 总多酚 响应面法 钙沉淀 

分 类 号：TQ028.8[化学工程] TQ914.1