酵母菌葡萄糖耐量因子的纯化  

Purification of Glucose Tolerance Factor(GTF) from High-Chromium Yeast

作　　者：孔凡华[1] 刘鹭[2] 张书文[2] 芦晶[2] 李红娟[2] 薛海晓 赵丽丽[2] 周志江[1] 吕加平[2] 

机构地区：[1]天津大学化工学院,天津300072 [2]中国农业科学院农产品加工研究所,北京100193

出　　处：《食品科学》2014年第21期1-4,共4页Food Science

基　　金：国家自然科学基金面上项目(31371763)

摘　　要：以高铬酵母菌粉为研究对象,采用温和的纯化方法,通过氨水提取高铬酵母菌粉中的酵母菌葡萄糖耐量因子(glucose tolerance factor,GTF),联用SuperdexTM75和Sephadex G25凝胶过滤层析对提取物进行粗分离,然后用反相高效液相色谱对提取物进行再分离,从而对GTF进行高效纯化研究。将GTF纯化品作用于胰岛素抵抗型3T3-L1脂肪细胞,通过检测细胞葡萄糖消耗量,分析GTF纯化品对细胞葡萄糖代谢调节活性。研究表明,GTF纯化品可以有效提高胰岛素抵抗型3T3-L1脂肪细胞葡萄糖的消耗。可见,经过多步柱层析纯化后,可以得到具有葡萄糖代谢调节活性的GTF纯化品。In this study, a mild extraction procedure was used to purify glucose tolerance factor(GTF) from high-chromium yeast cells. GTF was extracted by ammonia from high-chromium yeast cells. SuperdexTM 75 and Sephadex G25 gel filtration chromatography were adopted to purify the extracts. Reverse phase high-performance liquid chromatography(RP-HPLC) was used for further purification. The glucose metabolism regulating activity of GTF was evaluated in insulin-resistant 3T3-L1 adipocytes. This research showed that through multiple steps of column chromatography, the pure GTF was obtained, which could improve glucose metabolism in insulin-resistant 3T3-L1 adipocytes.

关 键 词：酵母菌葡萄糖耐量因子 高铬酵母粉 纯化 胰岛素抵抗型3T3-L1脂肪细胞 葡萄糖代谢 

分 类 号：TQ464[化学工程—制药化工] R587.1[医药卫生—内分泌]