肝癌患者乙型肝炎病毒X基因变异的研究  

Study on X gene mutation of hepatitis B virus from patients with hepatocellular carcinoma

作　　者：代志琰[1] 徐启桓[1] 李刚[1] 马会慧[1] 汤正好[1] 舒欣[1] 姚集鲁[1] 

机构地区：[1]中山大学附属第三医院传染科,广东省广州市510630

出　　处：《世界华人消化杂志》2003年第9期1349-1352,共4页World Chinese Journal of Digestology

基　　金：广东省自然科学基金;No.994030

摘　　要：目的:探讨乙型肝炎病毒(HBV)X基因变异在肝癌发生中的作用.方法:应用聚合酶链反应(PCR)技术从5例肝细胞癌患者及12例慢性乙型肝炎患者血清中扩增X基因,然后将纯化的PCR产物进行序列测定,对获得的序列进行变异性及同源性分析.结果:肝细胞癌患者中得到的HBV X基因序列与Genebank中多个已发表的HBV各血清亚型序列进行同源性比较,发现核苷酸同源性在89—96％之间,血清亚型以adw为主.与非肝癌组的比较发现X基因序列的替换突变中存在4种形式.6个位点的变异仅出现于肝癌组,12个热点变异多见于肝癌组而少见于非肝癌组.结论:X基因序列的突变与肝细胞癌的发生有关。AIM: To study the association of mutations of hepatitis B virus(HBV) X gene with hepatocellular carcinoma(HCC). METHODS: The complete X region of HBV from the serum of 5 patients with HCC and 12 patients with chronic hepatitis B(CH) was amplified by PCR and then the purified PCR products were sequenced. RESULTS: A comparison of 492bp nucleotide sequence of the HBV X gene from 5 HCC patients with that of the several previously reported isolates of different serotype in Genebank showed that the nucleotide homology was 89-96 % and most of the strains were similar to serotype adw. The number of nucleotide changes in the X gene of HBV from HCC group (19.4±12.1) was significantly greater than that from the CH group (4.4±2.4, P=0.00093).There were 4 models of substitutions in X gene and 6 hot spots found in HCC group and 12 hot spots found in HCC group. CONCLUSION: The mutations of HBV X gene may be closely associated with the development of HCC.

关 键 词：肝癌 乙型肝炎病毒 X基因 基因变异 聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤] R373.21[医药卫生—临床医学]