人γ-干扰素基因工程菌培养条件的研究  

Research on cultivation conditions of engineering E.coli expressing recombinant human interferon-γ

作　　者：靳挺[1] 关怡新[1] 姚善泾[1] 

机构地区：[1]浙江大学化学工程与生物工程学系,浙江杭州310027

出　　处：《浙江大学学报（工学版）》2003年第6期724-728,共5页Journal of Zhejiang University：Engineering Science

基　　金：国家自然科学基金资助项目(20076042);浙江省自然科学基金资助项目(201099).

摘　　要：对表达重组人γ-干扰素的基因工程菌的培养条件进行了较详细的研究.确定了最佳的培养及诱导时间,并利用正交试验设计,对影响工程菌生长和重组人γ-干扰素表达的条件如培养基中葡萄糖、蛋白胨、酵母提取物的量及摇床转速、装液量、接种量、pH等进行了优化.结果表明,在优化条件下,表达的γ-干扰素包涵体占菌体总蛋白的60%以上,每升发酵液可获得包涵体约为1.6g.Cultivation conditions of the genetic engineering E.coli (pBV220IFN-γ DH5α)which expressed recombinant human interferon-γ were investigated. The optimum culture and induction time was determined. Orthogonal experiment design was employed to optimize experiment conditions (such as components of culture medium (glucose, peptone, yeast extraction),shaking speed, medium volume, inoculum concentration and pH) affecting its growth and expression of recombinant human interferon-γ.Results shows that rhu-interferon-γ is expressed with 60% of the total cell protein; and 1.6 g inclusion bodies per liter culture medium are obtained under optimized conditions.

关 键 词：重组人Γ-干扰素 培养条件 高效表达 基因工程菌 包涵体 发酵 

分 类 号：TQ929.1[轻工技术与工程—发酵工程]