脂溶性茶多酚中的主要活性组分及对人卵巢癌HO-8910细胞株的体外抑制活性  

The Main Active Component of Lipophilic Tea Polyphenols and in vitro Inhibition Activity on Ovarian Cancer Cells HO-8910

作　　者：陈平[1] 钟建华[1] 孙东[2] 

机构地区：[1]浙江大学化学系,浙江杭州310028 [2]温州医学院,浙江温州325000

出　　处：《茶叶科学》2003年第2期115-118,104,共5页Journal of Tea Science

摘　　要：利用高速逆流色谱对脂溶性茶多酚中的主要活性组分进行分离和纯化,获得了一种新的单取代的长碳链脂溶性儿茶素表没食子儿茶素-3-O-没食子酸-4'-棕榈酸酯,并对其分子结构进行了元素分析、IR、MS和1H-NMR等表征。药理学实验考察并比较了脂溶性的表没食子儿茶素-3-O-没食子酸-4'-棕榈酸酯、水溶性的绿茶多酚和脂溶性茶多酚对人卵巢癌HO-8910细胞株的体外抑制活性。结果表明,单取代的EGCG棕榈酸酯的活性比脂溶性茶多酚强,而与绿茶多酚相当。A single-substitution long-chain lipophilic catechin was isolated and purified from lipophilic tea polyphenols by high-speed countercurrent chromatography (HSCCC). Its molecular structure was elucidated as epigallocatechin-3-O-gallate-4'-palmitate by elemental analysis, IR, MS and 1H-NMR. The in vitro inhibition activity of the lipid-soluble epigallocatechin-3-O-gallate-4'-palmitate on ovarian cancer cells HO-8910 was investigated and compared with that of green tea polyphenols and lipophilic tea polyphenols. The results demonstrated that the inhibition activity of the single-substitution EGCG palmitate was almost corresponding to green tea polyphenols, and more effective than lipophilic tea polyphenols.

关 键 词：脂溶性茶多酚 活性组分 人 卵巢癌 高速逆流色谱 分子结构 药理学 茶学 EGCG棕榈酸酯 体外抑制活性 

分 类 号：R737.31[医药卫生—肿瘤]