半纤维素制备方法的改进及其应用  

Improved Extraction Method for Hemicellulose and Its Application

作　　者：王雪鹏[1] 管斌[1] 汤海青[1] 郭姗娜[1] 

机构地区：[1]中国海洋大学水产学院,山东青岛266003

出　　处：《无锡轻工大学学报（食品与生物技术）》2003年第6期85-88,共4页Journal of Wuxi University of Light Industry

摘　　要：对玉米芯中半纤维素的提取方法及条件进行改进,将蒸煮法与碱法提取相结合有效提高了半纤维素的提取率和提取纯度.以提取的半纤维素作为选择性碳源,可用来制备分离筛选木聚糖酶产生菌的分离培养基;探讨了半纤维素添加方式及添加量对培养基产透明圈效果的影响.In this paper,the extracting methods and conditions of hemicellulose from corncob were improved,the ratio and purity of extraction were effectively raised by integration of cooking with alkalitreatment.The extracted hemicellulose were used as selective carbon source for selective medium to separate and screen xylanaseproducing strains. The addition manner and dose of hemicellulose which influenced transparent zones caused by the strains on the plate were studied.This selective medium could produce obvious transparent zones and the xylanase activity could be identified by these transparent zones elementarily. In this way, the efficiency of primary screening were effectively improved.

关 键 词：玉米芯 半纤维素 提取方法 提取率 木聚糖酶产生菌 分离 培养基 

分 类 号：TS201[轻工技术与工程—食品科学]