固相微萃取-气相色谱/质谱分析生姜的挥发性成分(英文)  

Gas Chromatography-mass Spectrometry Coupled with Solid-phase Microextraction for Determination of Volatile Constituents of Chinese Ginger

作　　者：宋国新[1] 邓春晖[2] 吴丹[1] 胡耀铭[1] 

机构地区：[1]复旦大学分析测试中心,上海200433 [2]复旦大学化学系,上海200433

出　　处：《复旦学报（自然科学版）》2003年第6期939-944,949,共7页Journal of Fudan University：Natural Science

基　　金：Thestart upscientificresearchfundforyoungteachers

摘　　要：采用固相微萃取(SPME)技术结合气相色谱 质谱(GC MS)分析了生姜中的挥发性化合物.用膜厚为100μm的聚二甲基硅氧烷纤维头萃取新鲜生姜中的挥发性化合物,纤维头在温度为250℃气化室进样口脱附3min.通过顶空SPME GC MS方法从生姜中共分离和鉴定出36个化合物.其中主要的化合物为α 水芹烯,莰烯,里哪醇,香叶醛,姜烯,倍半水芹烯,橙花醛,α 没药烯,α 姜黄烯,由色谱峰的峰面积比例计算出各个化合物的相对含量.研究和讨论了影响SPME条件的参数,包括吸附时间、温度、脱附时间等.Gas chromatography-mass spectrometry combined with solid-phase microextraction (SPME-GC-MS) was developed for the determination of volatile compounds of ginger. The volatile constituents of fresh ginger were extracted by SPME with a 100 μm polydimethylsiloxane fiber. The fibers were desorbed in a GC injection liner at 250 ℃ for 3 min. With headspace SPME-GC-MS analysis, thirty-six peaks in ginger were separated and identified by mass spectrometry. The volatile compounds included α-phellandrene, camphene, linalool, geranial, zingiberene, sesquiphellandrene, neral, α-bisabolene, α-curcumene etc and their relative contents were calculated on basis of peak area ratio. SPME extraction conditions including time, temperature and desorption time were investigated.

关 键 词：生姜 挥发性化合物 气相色谱-质谱 固相微萃取 中药 

分 类 号：R284.1[医药卫生—中药学] O657.63[医药卫生—中医学]