D-葡萄糖串联发酵生产2-酮基-L-古龙酸的工艺条件  

THE TECHNOLOGICAL CONDITIONS OF PRODUCING 2-KETO-LGULONIC ACID FROM D-GLUCOSE BY DIRECT FERMENTATION

作　　者：张刚[1] 兰先德[1] 范小兵[1] 韩志华[1] 尹光琳[2] 马志方[2] 董文玲[2] 

机构地区：[1]上海交通大学生物科学与技术系,上海200030 [2]中国科学院上海生物工程研究中心,上海200233

出　　处：《微生物学通报》1992年第2期78-81,共4页Microbiology China

摘　　要：研究了在10L发酵罐中D-葡萄糖串联发酵生产维生素C前体——2-酮基-L-古龙酸的发酵工艺条件。第一步发酵采用欧文氏菌(Erwinia sp.)的突变株SCB247,培养36小时,可将D-葡萄糖转化成中间体2,5-二酮基-D-葡萄糖酸,在发酵液中约累积180mg/ml。第二步发酵采用棒状杆菌(Corynebacterium sp.)SCB3058,可将2,5-二酮基-D-葡萄糖酸专一性地还原生成2-酮基-L-古龙酸。在细胞生长进入对数生长期后期时,加入经十二烷基硫酸钠处理的第一步发酵液,约50小时以后,在发酵液中可累积35mg/ml的2-酮基-L-古龙酸。用10L发酵罐进行五批实验,结果表明,从D-葡萄糖到2-酮基-L-古龙酸的平均转化率为56.3mol%。2-Keto-L-gulonic acid (2-KLG), the precurcor of L-ascorbic acid synthesis, was prepared directly from D-glucose by tandem fermentation. In the first step fermentation Erwinia sp. SCB 247 translated D-glucose to 2,5-diketo-D-gluconate (2,5-DKG), which accumenlated 180 mg 2,5-DKG per ml in the broth. In the second step fermentation Corynebacterium sp. SCB 3058 reduced 2,5-DKG to 2-KLG, which accumulated 35 mg 2-KLG per ml in the broth. This reductive fermentation was obtained under aerobic conditions by adding a hydrogen donor such as glucose.The average yield of five batches fermentation was 56.3 mol%, from D-glucose to 2-KLG in 10 L fermentor.

关 键 词：2-酮基-L-古-酸 维生素C 发酵 

分 类 号：TQ921.7[轻工技术与工程—发酵工程]