微菌落滤膜法快速定量检测饮料中酵母  

A MEMBRANE MICROCOLONY COUNT METHOD FOR RAPID DETERMINATION OF THE AMOUNT OF YEAST IN BEVERAGES

作　　者：刘军[1] 叶梅君[2] 裴晓方[2] 许欣[2] 

机构地区：[1]深圳市卫生防疫站 [2]华西医科大学公共卫生学院医学检验教研室

出　　处：《现代预防医学》1992年第1期8-9,共2页Modern Preventive Medicine

摘　　要：本文介绍利用混合纤维素滤膜过滤饮料样品,培养后对其上生长的微菌落进行染色和计数,定量检测饮料中酵母。14份可乐样品在滤膜上的微菌落计数与相应平板培养法计数之间相关关系为(r=0.96,P<0.01),本法迅速,不到12小时报告结果,简便,适于检测污染酵母浓度高于10~3CFU/ml的饮料。A membrane microcolony count method was developed to measure the amount of yeast in beverages. A specific volume of the beverage sample was filtered by suction through a cellulose mixed membrane filter (pore diameter 5 μm). The filter was placed on the agar media and incubated for a few hours, followed by staining and counting with the light microscope. The amount of yeast in 14 Cola samples were counted by using this method and the plate count method. The results correlated well(γ=0.96, P<0.01). The microcolony count method is rapid, taking less than 12h, simple and suitable for beverages containing above 10~3 CFU yeast per ml.

关 键 词：微菌落 酵母 饮料 

分 类 号：R155.59[医药卫生—营养与食品卫生学]