利用谷物进行类胡萝卜素固态发酵  

Solid-state Fermentation of Grains for Carotenoid Production

作　　者：徐军[1] 崔丽霞[1] 韩建荣[1] 

机构地区：[1]山西大学生命科学与技术学院

出　　处：《食品与发酵工业》2004年第1期5-8,共4页Food and Fermentation Industries

基　　金：国家自然科学基金资助项目 (No 3 0 0 70 0 2 1 )

摘　　要：研究了 7种谷物培养基及相关因素对青霉PT95菌株进行类胡萝卜素固态发酵的影响作用。结果表明 ,谷物培养基的组成对PT95菌株在固态发酵条件下的菌核生物量和菌核中的类胡萝卜素含量都有明显的影响 ;接种方式和接种量能明显影响谷物培养基上的菌核生物量 ,但对菌核中的类胡萝卜素含量没有明显的影响 ;在谷物培养基里添加麸皮有利于菌核的形成。在选择最佳接种方式、接种量 ,并在培养基里添加 2 0 %麸皮 (干重 )的固态发酵条件下 ,大米培养基上得到的菌核生物量最高 ,达到 1 5 0 0 g/1 0 0 g(干料 ) ;荞麦培养基上 ,菌核中的类胡萝卜素含量最高 ,达到82 6μg/g(干菌核 ) ;而在谷子培养基上 ,类胡萝卜素产率最高 ,达到 1 1 45 7μg/1 0 0 g(干料 )。Various grains and inocula were investigated for carotenoid production by solid-state fermentation using Penicillium sp. PT95. The millet medium was more effective to the sclerotia growth of strain PT 95 and carotenoid production than other grain media. An inoculum in the form of sclerotium may obtain higher sclerotia biomass than either a spore inoculum or a mycelial pellet inoculum. Supplementing wheat bran to grain medium favored the formation of sclerotia. However, neither the inoculum type nor the supplementing of wheat bran resulted in a significant change in content of carotenoid in sclerotia. The effect of wheat brain in grain media with the ratio of wheat bran to grain 1∶4 (w/w, dry basis) was investigated. It is indicated that the medium consisting of rice and wheat bran gave the highest sclerotia biomass (15.10g/100g grain); the medium consisting of buckwheat and wheat bran gave the highest content of carotenoid in sclerotia (826μg/g dry sclerotia) and the medium consisting of millet and wheat bran gave the highest carotenoid yield (11457μg/100g grain).

关 键 词：类胡萝卜素 固态发酵 青霉PT95菌株 谷物培养基 菌核生物量 接种方式 产率 

分 类 号：TQ920[轻工技术与工程—发酵工程]