水相酶解法菜籽蛋白提取液超滤工艺研究  

Research on Ultrafiltration Technology for Rapeseed Protein after Aqueous Enzymic Hydrolysis

作　　者：刘志强[1] 曾云龙[1] 吴苏喜[2] 李会东[1] 

机构地区：[1]湖南科技大学生物与环境工程系,湖南湘潭411201 [2]长沙理工大学生物工程系,湖南长沙410120

出　　处：《中国粮油学报》2004年第1期52-56,共5页Journal of the Chinese Cereals and Oils Association

基　　金：湖南省自然科学基金资助项目(0 2JJY50 0 1 )

摘　　要：对水相酶解法提取菜籽油与菜籽蛋白所得菜籽蛋白液进行超滤处理 ,研究超滤条件对超滤膜透过速率的影响 ,得出操作压力、温度、料液的 pH、料液蛋白质浓度影响较大。试验结果表明 ,控制操作压力 0 .2 0～ 0 .30MPa ,温度 5 0℃ ,料液 pH9～ 10时效果较好。采用超滤工艺 ,所得菜籽蛋白不含毒素、纯度及回收率在 90 %以上 。This research intends to discover the effect of ultrafiltration on the ratio of permeating flux for UF membrane by applying the technology of ultrafiltration to the protein yielded after aqueous enzymic extraction for rapeseed oil and protein. It verifies that the pressure and temperature of operation, and the pH and thickness of material liquid have obvious effects on the ratio of permeating flux for UF membrane.The optimum conditions are supposed to be as follows: the operating pressure, 0.20-0.30Mpa; temperature, 50℃; and pH 9-10. It shows that the protein yielded by adopting the technology of ultrafiltration contains no toxin, that the purity and recycling rate of protein exceeds 90%, and that the functional properties of protein are much better in comparison with protein by acid subside.

关 键 词：水相酶解法 菜籽蛋白 超滤工艺 菜籽油 超滤膜 蛋白质浸提液 

分 类 号：TS225.14[轻工技术与工程—粮食、油脂及植物蛋白工程] TS229[轻工技术与工程—食品科学与工程]