超声提取气相色谱法快速测定食品中甜蜜素含量的研究  

Study on ultrasonic extraction of sodium cylamate from foods and rapid determination by gas chromatography

作　　者：白艳玲[1] 王丽玲[1] 

机构地区：[1]珠海市疾病预防控制中心,广东珠海市519000

出　　处：《中国热带医学》2004年第2期190-191,共2页China Tropical Medicine

摘　　要：目的　建立超声波提取法测定食品中的甜蜜素。　方法　食品中的甜蜜素经超声波提取后、衍生化、用气相色谱法测定。　结果　方法的检出限为 0 .0 18g/kg(S/N =3 ) ;甜蜜素质量浓度在 0 0 5g/L～ 16 0g/L范围内具有良好的线性关系 ,相关系数r =0 .99999。样品测定的RSD为 4 90 %～ 6 70 % (n =6) ,加标回收率为 94 0 0 %～ 10 3 0 0 % ,与研磨提取法比较无显著性差异 (P >0 .0 5 )。　结论　本方法简便、快速、准确 ,适用于批量样品的测定。Objective To develop a method for ultrasonic extraction of sodium cyclamate from foods and rapid determination by gas chromatography. Methods Sodium cyclamate in foods was ultrasonically extracted,derivatized by sodium nitrite and determined by gas chromatography. Results The detection limit of the method was 0.018g/kg,the calibration curve of sodium cyclamate showed good linearity in the range of 0.05g/L～16.0g/L with r=0.99999.The RSDs were from 4.90% to 6.70%(n=6),and the added standard recovery rates were from 94 00% to 103 00%.There was no significant differences between untrasonic extraction and pestle extraction (P>0.05). Conclusion This method is simple,rapid,accurate and suitable for determination of wholesome samples.

关 键 词：气相色谱法 含量测定 食品 甜蜜素 研磨提取 超声波提取 

分 类 号：R155.5[医药卫生—营养与食品卫生学]