葡萄胎的遗传学分类与病理分类的关系  

The study of the relationship between the classification of geneti typing and the pathology of hydatidiform mole

作　　者：张小为[1] 朱浩彬[1] 高雅琴[2] 武淑英[1] 高荣莲[1] 韩劲松[1] 王秀云[1] 王蕊[3] 郭红燕[1] 赵扬玉[1] 赵文秋[1] 

机构地区：[1]北京大学第三医院妇产科,北京100083 [2]大庆油田总医院妇产科 [3]天坛医院妇产科

出　　处：《中国生育健康杂志》2004年第2期89-91,共3页Chinese Journal of Reproductive Health

基　　金：国家自然科学基金 (批准号 :39770 76 9);北京大学医学部"十五"; 2 1 1工程项目基金 (编号 :50 8)

摘　　要：目的　探讨葡萄胎的复合微卫星 DNA序列多态性的遗传学分类与病理学分类的关系。　方法　采用 9个位点 (F13 A0 1、 FESFPS、 VWA、 CSF1P0、 TPOX、 TH0 1、 D16S53 9、 D7S82 0、D13 S3 17)复合微卫星序列 -PCR方法对 47例葡萄胎病例标本进行分析 ,与病理学分类结果进行比较和统计分析。　结果　 47例葡萄胎病例中 ,3 3例为遗传学完全性葡萄胎 ,14例为遗传学部分性葡萄胎 ,40例为病理学完全性葡萄胎 ,7例为病理学部分性葡萄胎 ,采用 Kappa一致性检验 ,结果 Kappa= 0 .3 4 6± 0 .14 8(P<0 .0 1)。　结论　复合 STR多态性分析可用于葡萄胎的遗传学分类 ;Objective To investigate the feasibility of using STR polymorphisms at the study of genetic typing of hydatidiform mole and to study the relationship between the classification of genetics and pathology.Methods All 47 specimens were typed by multiplex STR PCR(9 loci) and compared with pathology classifications. Results The cases of DNA from paternal origin only were found in 33 cases,and were found from both parents in 14 cases.In all the cases on the basis of pathological characteristics,there were 40 cases of complete hydatidiform mole (CHM) and 7 cases of partial hydatidiform mole (PHM). Conclusion There is no correlation between classification of pathology and molecular genetics for hydatidiform mole;STR polymorphisms analyzing can be used in genetics material origin determination of hydatidiform mole.

关 键 词：葡萄胎 遗传学 病理分类 DNA序列 微卫星序列 短串联重复序例 

分 类 号：R737.33[医药卫生—肿瘤]