反相高效液相色谱法监测蔗糖-6-乙酸酯合成  

Study on the method of reversed-phase high performance liquid chromatography used for monitoring synthesis of sucrose-6-acetate

作　　者：张英[1] 粟晖[1] 韦异[1] 廖敬阳[1] 郭霖[1] 

机构地区：[1]广西工学院轻工化工系,广西柳州545006

出　　处：《化学研究与应用》2004年第2期245-247,共3页Chemical Research and Application

基　　金：广西省科技厅自然科学基金项目 (桂科自 0 0 660 0 1) ;广西工学院科学研究基金资助项目 (0 0 2 19)

摘　　要：A method was established of reversed-phase high performance liquid chromatography for the determination of sucrose-6-acetate.In the method,SupelcosilTMLC-18（250mm×4.6mm i.d.5μm）was used as chromatographic column and V（acetonitrile）∶V（methanol）∶V（water）=10∶10∶80 as mobile phase.The linear ranges was 0.5mg/L～900mg/L.The lower limit of detection of surose-6-acetate was 0.2mg/L.The recovery of the method was 98.53% and the relative standard deviations was 0.95%.By the method,the reaction raw materials,the reaction mediates and the reaction products could be separated effcctively,and the reaction progress could also be indicated directly.A method was established of reversed-phase high performance liquid chromatography for the determination of sucrose-6-acetate.In the method,Supelcosil^(TM)LC-18(250mm×4.6mm i.d.5μm)was used as chromatographic column and V(acetonitrile)∶V(methanol)∶V(water)=10∶10∶80 as mobile phase.The linear ranges was 0.5mg/L～900mg/L.The lower limit of detection of surose-6-acetate was 0.2mg/L.The recovery of the method was 98.53% and the relative standard deviations was 0.95%.By the method,the reaction raw materials,the reaction mediates and the reaction products could be separated effcctively,and the reaction progress could also be indicated directly.

关 键 词：蔗糖-6-乙酸酯 合成 反相高效液相色谱法 三氯蔗糖 中间体 食品添加剂 甜味剂 

分 类 号：TS202.3[轻工技术与工程—食品科学]