嗜酸乳杆菌增菌培养研究  

Study on the growth promoting culture of Lactobacillus acidophilus

作　　者：陈小梅[1] 张迅捷[1] 罗建玲[1] 吴超[1] 

机构地区：[1]福建华南女子学院,福州350007

出　　处：《四川食品与发酵》2004年第2期40-42,共3页Sichuan Food and Fermentation

基　　金：福建省自然科学基金资助项目(B0010033)

摘　　要：对培养嗜酸乳杆菌的基础培养基进行筛选,确定为11%NFM培养基.采用正交试验对增殖因子进行优化,得到最优增菌培养基:基础培养基+5%番茄汁+10%萝卜汁+0.1%肝浸汁+5%啤酒.以最优增菌培养基测定生长曲线、pH值和滴定酸度的变化,确定增菌培养终止时间为10hr,此时活菌数达7.49×109cfu/ml.11% NFM medium was founded by screening several radical media to cultivate Lactobacillus acidophilus. Based on the 11% NFM medium, the optimal medium was obtained by orthogonality test consisted of tomato juice 5%,carrot juice 10%, liver infusion 0. 1%, beer 5%. Growth curve with optimized stimulated culture was determined by following the change of pH and titratable acidity. According to the growth curve. the terminal time was in 10 hours and Lactobacillus acidophilus got to 7. 49×10~9cfu/ml.

关 键 词：嗜酸乳杆菌 增菌培养 筛选 基础培养基 正交试验 

分 类 号：TS201.3[轻工技术与工程—食品科学]