外源添加ATP对K.pneumoniae菌体生长和产物合成的影响  

Effect of Addition of ATP on Cell Growth and 1,3-Propanediol Production of K.pneumoniae

作　　者：饶治[1] 张延平[1] 沈金玉[1] 李春[1] 曹竹安[1] 

机构地区：[1]清华大学化学工程系生物化工研究所,北京100084

出　　处：《精细化工》2004年第6期413-417,共5页Fine Chemicals

基　　金：十五"国家科技攻关计划项目(编号:2001BA708B01-04)~~

摘　　要：基于K.pneumoniae生成1,3 丙二醇过程中甘油脱水酶催化失活与复活的特性,在发酵培养基中添加不同质量浓度的三磷酸腺苷(ATP),考察ATP对菌体生长、1,3 丙二醇(1,3 PD)、其他副产物合成的影响。实验结果表明:添加ATP抑制菌体生长,加入质量浓度为0 4g/LATP,OD值比参照低53%;添加ATP促进单位细胞合成1,3 丙二醇,加入质量浓度为0 4g/LATP,1,3 丙二醇质量浓度/OD值比参照高90%;添加ATP能提高甘油到1,3 丙二醇转化率,加入0 8g/LATP,甘油到1,3 PD的最大转化率达到0 76mol/mol。Based on the characteristics of catalyst inactivation and reactivation of glycerol dehydratase during production of 1,3-propanediol(1,3-PD),ATP is added in fermentation medium of Klebsiella pneumoniae to study the effect of ATP on cell growth,1,3-PD and other by-products production.The results show that addition of ATP has an inhibitory effect on cell growth:adding 0.4 g/L ATP,OD is 53% lower than control.Addition of ATP increases the production of 1,3-PD per cell:adding 0.4 g/L ATP,1,3-PD concentration/OD is 90% higher than control.Addition of ATP can prompt molar yield of 1,3-PD:adding 0.8g/L ATP,the maximal molar yield of 1,3-PD reaches 0.76 mol/mol.

关 键 词：1 3-丙二醇 ATP 甘油 Klebslella PNEUMONIAE 3-羟基丙醛 

分 类 号：TQ923[轻工技术与工程—发酵工程]