猕猴桃果实采后成熟过程中糖代谢及其调节  

Sugar Metabolism and Its Regulation in Postharvest Ripening Kiwifruit

作　　者：张玉[1] 陈昆松[1] 张上隆[1] 王建华[2] 

机构地区：[1]浙江大学果实分子生理与生物技术实验室农业部园艺植物生长发育与生物技术重点开放实验室,华家池校区杭州310029 [2]浙江省武义县农业局,浙江武义321200

出　　处：《植物生理与分子生物学学报》2004年第3期317-324,共8页Journal Of Plant Physiology and Molecular Biology

基　　金：国家重点基础研究发展规划项目(973项目)(No.G2000046806);国家自然科学基金项目(No.30270917);浙江省自然科学基金重点项目(No.ZD0004)资助

摘　　要：20℃下采后猕猴桃果实中淀粉酶活性快速上升于果实软化启动阶段,随着果实进入快速软化阶段,淀粉迅速水解,葡萄糖和果糖快速积累,SPS活性增加,酸性转化酶活性下降,蔗糖积累;至果实软化后期,SPS活性降低,蔗糖含量下降。AsA和低温可抑制淀粉酶活性、己糖积累、SPS活性上升和酸性转化酶活性下降,延缓蔗糖积累,相反,乙烯则可促进淀粉酶活性,加速淀粉降解和己糖积累进而直接或间接增加SPS活性,促使蔗糖积累。采后猕猴桃果实的SPS活性变化中有己糖激活效应和蔗糖反馈抑制效应。AsA、低温和乙烯等对糖代谢的调节主要是通过对SPS活性的影响而实现的。Kiwifruit (Actinidia deliciosa cv. Bruno) was used to investigate starch and sugar metabolism and the mechanisms of regulation by acetylsalicylic acid (AsA 1.0 mmol/L, pH 3.5), low temperature (0℃) and ethylene (100 μL/L) treatments. There was an increase in amylase activity at the initial stage followed by dramatical decrease in starch content and a rapid increase in hexose content at the rapid stage of fruit ripening and softening (Figs.1-3), which was associated with an increase in SPS activity, a decrease in acid invertase activity (Fig.4), and the accumulation of sucrose (Fig.3). AsA and low temperature treatments inhibited the amylase activity, slowed down the hydrolysis of starch (Figs.2, 6) and the accumulation of hexoses (Figs.3, 8), suppressed the rise of SPS activity and the decline of acid invertase activity (Figs. 4, 10) in the ripening fruit (Figs.l, 5). The accumulation of sucrose was delayed by AsA and low temperature treatments (Figs.3,8). However, eth-ylene application induced amylase activity, accelerated starch hydrolysis (Fig.7), and raised the hexose content (Fig.9). The SPS activity also increased and the sucrose accumulated in the presence of ethylene (Figs.9,11). It is suggested that the SPS may play a key role in sugar metabolism of postharvest kiwifruit, and it could be activated by hexose and feedback-inhibited by sucrose. AsA, low temperature and ethylene treatments regulate sugar metabolism probably through influencing the SPS activity.

关 键 词：猕猴桃果实 乙酰水杨酸 乙烯 低温 糖代谢 

分 类 号：S663.4[农业科学—果树学]