离子注入纤维分解细菌酶活性提高机理研究  

Induced Mechanism of Fibre Decomposition Bacterium by Low-Energy Ions

作　　者：陈燕勤[1] 曾宪贤[1] 

机构地区：[1]新疆大学离子束生物工程中心

出　　处：《种子》2004年第5期3-5,17,共4页Seed

基　　金：国家自然科学基金项目 (1 0 1 650 0 1 );"十五"国家科技攻关计划 (2 0 0 1 BA30 2 B) ;新疆自然科学基金项目 (2 0 0 0 1 );乌鲁木齐市重大科研基金项目 (Y0 0 1 0 )

摘　　要：纤维分解细菌 N+ 离子注入前后菌株的培养滤液经 (NH4 ) 2 SO4 沉淀 ,Sephadex G- 2 5脱盐 ,Sephadex G- 75凝胶过滤及 DEAE- Sephadex A- 5 0离子交换层析。发现经 N+离子处理的菌株酶系发生变化 ,尤其是 β-葡萄糖苷酶组分增加很多 ,其余组分也发生了相应变化。经 N+ 处理后各酶组分与 DEAE- Sephadex A- 5 0胶结合能力发生了变化 ,Hy2 所需Na Cl浓度主要为 0 .5 0 mol/ L ,而经 N+ 处理后变为从 0 .30～ 0 .5 0 mol/ L。Hy 2 and Hy 2-3311's filtrate precipitate by (NH 4) 2SO 4, Sephadex G-25 desalination, Sephadex G-75 column chromatography and ion exchange chromatography on DEAE-Sephadex A-50. The results shown that cellulase components changed by low-energy ions inducing, especially β-glucosidase components were increased and other components were changed as well. It is discovered that capacity of cellulase combine with DEAE-Sephadex A-50 was changed and that eluted with NaCl, the Hy 2-3311needs limit is 0.30~0.50 mol/L, but Hy 2 is only 0.5 mol/L.

关 键 词：离子注入 纤维分解细菌 酶活性 Β-葡萄糖苷酶 纤维素酶 CMC酶 

分 类 号：Q55[生物学—生物化学]