菹草红色类胡萝卜素的拉曼光谱特性研究  

RAMAN MICRO-SPECTROSCOPY OF RED CAROTENOIDS FROM POTAMOGETON CRISPUS L.

作　　者：王海滨[1] 任丹丹[1] 刘良忠[1] 石嘉怿[1] 张声华[1] 

机构地区：[1]华中农业大学食品科技学院

出　　处：《水生生物学报》2004年第4期380-384,共5页Acta Hydrobiologica Sinica

基　　金：教育部 2 0 0 2年度博士点基金资助项目 (No .2 0 0 2 0 5 0 40 0 8)

摘　　要：用MgO 硅藻土 (1∶2 ,w/w)柱层析和硅胶G薄层层析 (TLC)从菹草中分离纯化了三种红色素r1、r2和r3,其极性大小排序依次为 :虾青素 >r1>r2 >r3>角黄素 >β 胡萝卜素。以虾青素和 β 胡萝卜素为对照 ,运用显微拉曼技术对它们作了测试分析研究。在拉曼光谱的“指纹区”(<180 0cm-1) ,r1、r2和r3都在 15 2 7cm-1处有最强的特征谱线 ,对应的是c=c伸缩振动 (υc=c) ,r1在 116 2cm-1、r2和r3在 115 7cm-1有较强的特征谱线 ,对应的是c c伸缩振动(υc c) ,r1、r2和r3在 10 0 7cm-1有较强的特征谱线 ,对应的主要是甲基与碳链之间的摇摆振动 ;r1、r2和r3与虾青素及 β 胡萝卜素在 2 0 0 0— 4 0 0 0cm-1区也有很好的对应关系。实验结果充分证明菹草红色素r1、r2和r3属于类胡萝卜素化合物 ,r1、r2和r3之间及与虾青素和 β 胡萝卜素的拉曼光谱都存在差异 ,说明它们的细微结构不同 ,其具体结构有待进一步研究。Potamogeton crispus L. is a kind of nature aquatic plant and widely dispersed over the world with important value in ecology and economy, it was reported that there were many nutritious elements such as proteins,fats, amino acids, fibres, Ca, P, vitamins etc. in it, and the utilizable value of feedstuffs has been understood preliminarily. The appearance that ducks fed with Potamogeton crispus could produce natural red-yolk eggs with good quality and price had been known long ago in Xiantao and Honghu area, Hubei province, the P. R. China, we have preliminarily found a high contents of carotenoids (especially a kind of red pigments) in Potamogeton crispus,the egg yolks color values could increase greatly when ducks were fed with it, and the red and some yellow pigments of the red-yolk eggs were in correspondence with the pigments in Potamogeton crispus by thin-layer chromatography, IR, and LC-MS, etc. Many researches showed that carotenoids not only was a kind of important natural pigments, but also possessed important biological functions including antioxidant activity, enhancing the immune system and the cell gap junctional communication, inducing cellular apoptosis, and regulating gene expression, so it is hot-point to find the biological resources abundant in carotenoids, we must make systematic studies on carotenoids of Potamogeton crispus so as to exploit this natural resources well. Three red pigments r1, r2 and r3 were extracted and separated by means of MgO-Diatomaceous Earth(1∶2,W/W) column and silica-gel G TLC from Potamogeton crispus L., the good separating solvent mixtures on the silica G TLC were:hexane-ethyl acetate-acetone-methanol=27∶4∶2∶2 (v/v/v/v), the R f of r1, r2 and r3 on the TLC were 0.32, 0.34 and 0.38, respectively, the arrangement in accordance with the polarity were astaxanthin>r1>r2>r3>canthaxanthin>β-carotene. Raman spectroscopy can provide detailed information on molecular vibrations, among naturally occurring chromophores, carotenoids have proved to be very suitable fo

关 键 词：菹草 红色素 类胡萝卜素 显微拉曼光谱 眼子菜科 

分 类 号：Q949.712.2[生物学—植物学]