Morphofunctional Properties of Human Platelets, Treated with Low Doses of Hydrogen Peroxide  

作　　者：Maksim S. Makarov Maya V. Storozheva Maksim S. Makarov;Maya V. Storozheva(Laboratory of Biotechnologies and Transfusiology, N. V. Sklifosovsky Research Institute for Emergency Medicine of Moscow Healthcare Department, Moscow, Russia)

机构地区：[1]Laboratory of Biotechnologies and Transfusiology, N. V. Sklifosovsky Research Institute for Emergency Medicine of Moscow Healthcare Department, Moscow, Russia

出　　处：《Journal of Biosciences and Medicines》2020年第9期125-130,共6页生物科学与医学（英文）

摘　　要：We studied morphofunctional properties of human platelets, treated with low doses of hydrogen peroxide (H₂O₂). It was shown that platelet incubation with 20 - 75 <em>μ</em>M H₂O₂ during 24 hours at 20<span style="white-space:nowrap;">&deg;</span>C - 22<span style="white-space:nowrap;">&deg;</span>C did not cause visible structural deficiencies in cells. Platelets maintained their adhesion activity and velocity of degranulation at 20 <em>μ</em>M H₂O₂. 30 - 60 μM H₂O₂ dose-dependently reduced lamella’s growth and allowed retaining granule bulk inside cytoplasm of spreading platelets. After 1 - 2 hours, the most effect of platelet granules’ stabilization was registered at samples, previously incubated with 60 μM H₂O₂. Adrenalin activation led to rapid degranulation of stabilized platelets, whereas lamella’s growth in activated platelets was low or medium.We studied morphofunctional properties of human platelets, treated with low doses of hydrogen peroxide (H₂O₂). It was shown that platelet incubation with 20 - 75 <em>μ</em>M H₂O₂ during 24 hours at 20<span style="white-space:nowrap;">&deg;</span>C - 22<span style="white-space:nowrap;">&deg;</span>C did not cause visible structural deficiencies in cells. Platelets maintained their adhesion activity and velocity of degranulation at 20 <em>μ</em>M H₂O₂. 30 - 60 μM H₂O₂ dose-dependently reduced lamella’s growth and allowed retaining granule bulk inside cytoplasm of spreading platelets. After 1 - 2 hours, the most effect of platelet granules’ stabilization was registered at samples, previously incubated with 60 μM H₂O₂. Adrenalin activation led to rapid degranulation of stabilized platelets, whereas lamella’s growth in activated platelets was low or medium.

关 键 词：PLATELETS GRANULES LAMELLA DEGRANULATION STABILIZATION 

分 类 号：TG1[金属学及工艺—金属学]