Effects of Dexamethasone, Clonidine, Tramadol and Nalbuphine on Fentanyl-Induced Hyperalgesia in Rats  

作　　者：Camila dos Santos Leite Naira Correia Cusma Pelógia Eliane Stevanato Marília Hidalgo Uchôas Marta Helena Rovani Pires Oscar César Pires Camila dos Santos Leite;Naira Correia Cusma Pelógia;Eliane Stevanato;Marília Hidalgo Uchôas;Marta Helena Rovani Pires;Oscar César Pires(Laboratory of Immunopharmacology and Molecular Biology, São Francisco University Medical School, Bragança Paulista, Brazil;Laboratory of Cell and Molecular Tumor Biology and Bioactive Compounds, São Francisco University Medical School, Bragança Paulista, Brazil;Laboratory of Pharmacology and Physiology, Medicine Department, Taubaté University, Taubaté, Brazil;Vera Cruz Hospital, Campinas, Brazil)

机构地区：[1]Laboratory of Immunopharmacology and Molecular Biology, Sã o Francisco University Medical School, Braganç a Paulista, Brazil [2]Laboratory of Cell and Molecular Tumor Biology and Bioactive Compounds, Sã o Francisco University Medical School, Braganç a Paulista, Brazil [3]Laboratory of Pharmacology and Physiology, Medicine Department, Taubaté University, Taubaté, Brazil [4]Vera Cruz Hospital, Campinas, Brazil

出　　处：《Journal of Biosciences and Medicines》2021年第12期87-97,共11页生物科学与医学（英文）

摘　　要：Opioids are drugs used to alleviate pain. However, studies have demonstrated that these drugs can cause an increase in pain sensitivity, which is called opioid-induced hyperalgesia. The objective of this study was to describe the effects of dexamethasone, clonidine, tramadol and nalbuphine on fentanyl-induced hyperalgesia in rats. After obtaining approval from the Committee for the Ethical Use of Animals (CEUA), 36 male Wistar rats were divided into 6 groups: Group 1 (GCSSL) wherein the rats received 1 ml 0.9% saline solution in two injections;Group 2 (GFTSL), received fentanyl at a dose of 100 ug<span style="white-space:nowrap;">&middot;</span>kg^-1 followed by 1 ml 0.9% saline solution via intraperitoneal;the remaining groups (3, 4, 5, 6) received fentanyl at a dose of 100 ug<span style="white-space:nowrap;">&middot;</span>kg^-1 following doses via intraperitoneal: Group 3 (GFTDX), dexamethasone at a dose of 1.0 mg<span style="white-space:nowrap;">&middot;</span>kg^-1;Group 4 (GFTCL), clonidine at a dose of 20 mg<span style="white-space:nowrap;">&middot;</span>kg^-1;Group 5 (GFTTR), tramadol at a dose of 50 mg<span style="white-space:nowrap;">&middot;</span>kg^-1, and Group 6 (GFTNB), nalbuphine at a dose of 5 mg<span style="white-space:nowrap;">&middot;</span>kg^-1. Under general anestesia using isoflurane, the animals were submitted to a surgical incision. Hyperalgesia was evaluated by applying Von Frey filaments at 2 hours after the incision and on the 1^st, 3^rd and 5^th days afterward. At 2 hours after the surgical procedure, there was lower intensity of pain in the fentanyl group (GFTSL) compared to the other groups, and on the fifth day there were no significant differences for pain intensity between groups. The results suggest the presence of fentanyl-induced hyperalgesia and efficacy in its reduction by dexamethasone, clonidine, tramadol and nalbuphine.Opioids are drugs used to alleviate pain. However, studies have demonstrated that these drugs can cause an increase in pain sensitivity, which is called opioid-induced hyperalgesia. The objective of this study was to describe the effects of dexamethasone, clonidine, tramadol and nalbuphine on fentanyl-induced hyperalgesia in rats. After obtaining approval from the Committee for the Ethical Use of Animals (CEUA), 36 male Wistar rats were divided into 6 groups: Group 1 (GCSSL) wherein the rats received 1 ml 0.9% saline solution in two injections;Group 2 (GFTSL), received fentanyl at a dose of 100 ug<span style="white-space:nowrap;">&middot;</span>kg^-1 followed by 1 ml 0.9% saline solution via intraperitoneal;the remaining groups (3, 4, 5, 6) received fentanyl at a dose of 100 ug<span style="white-space:nowrap;">&middot;</span>kg^-1 following doses via intraperitoneal: Group 3 (GFTDX), dexamethasone at a dose of 1.0 mg<span style="white-space:nowrap;">&middot;</span>kg^-1;Group 4 (GFTCL), clonidine at a dose of 20 mg<span style="white-space:nowrap;">&middot;</span>kg^-1;Group 5 (GFTTR), tramadol at a dose of 50 mg<span style="white-space:nowrap;">&middot;</span>kg^-1, and Group 6 (GFTNB), nalbuphine at a dose of 5 mg<span style="white-space:nowrap;">&middot;</span>kg^-1. Under general anestesia using isoflurane, the animals were submitted to a surgical incision. Hyperalgesia was evaluated by applying Von Frey filaments at 2 hours after the incision and on the 1^st, 3^rd and 5^th days afterward. At 2 hours after the surgical procedure, there was lower intensity of pain in the fentanyl group (GFTSL) compared to the other groups, and on the fifth day there were no significant differences for pain intensity between groups. The results suggest the presence of fentanyl-induced hyperalgesia and efficacy in its reduction by dexamethasone, clonidine, tramadol and nalbuphine.

关 键 词：HYPERALGESIA FENTANYL DEXAMETHASONE CLONIDINE TRAMADOL NALBUPHINE Rats 

分 类 号：R61[医药卫生—外科学]