The Combination of Artesunate and Paclitaxel in 1:1 Ratio Induces Apoptosis and Morphology Change on Human Prostate Cancer Cell Lines  

作　　者：Juan Fabian Taylor Pierce Shenell Brown Jazmyne Smith Dolapo Adedeji Gloria Payne Juan Fabian;Taylor Pierce;Shenell Brown;Jazmyne Smith;Dolapo Adedeji;Gloria Payne(Department of Natural Sciences, Elizabeth City State University, Elizabeth City, NC, USA;Department of Health & Human Studies, Elizabeth City State University, Elizabeth City, NC, USA)

机构地区：[1]Department of Natural Sciences, Elizabeth City State University, Elizabeth City, NC, USA [2]Department of Health & Human Studies, Elizabeth City State University, Elizabeth City, NC, USA

出　　处：《Pharmacology & Pharmacy》2023年第11期482-492,共11页药理与制药（英文）

摘　　要：The combination of Artesunate (ART) and Paclitaxel (PTX) in two human prostate cancer (PCa) cell lines (PC-3 and LNCaP) was evaluated to investigate the effects on proliferation, apoptosis and morphological changes. The half maximal inhibitory concentration (IC₅₀) values that were observed by ART and PTX on both LNCaP and PC-3 cell lines at 72-and 120-hour exposure were used to assess these effects. Early and late apoptosis was detected in Annexin V-FITC/PI assay revealed a shift in population of cells towards early and mid-apoptosis with ART + PTX than with ART and PTX individually. More effects were observed on LNCaP cell lines at both 72-hour and 120-hour exposure. The results for the Caspase 3/7 activity assay showed shift of viable population in all induced samples compared to control. Morphological changes occurred in both cell lines;this was validated in qualitative assessment when examined under the inverted microscope. These findings indicated that ART + PTX suppressed PCa cell proliferation in a dose- and time-dependent manner.The combination of Artesunate (ART) and Paclitaxel (PTX) in two human prostate cancer (PCa) cell lines (PC-3 and LNCaP) was evaluated to investigate the effects on proliferation, apoptosis and morphological changes. The half maximal inhibitory concentration (IC₅₀) values that were observed by ART and PTX on both LNCaP and PC-3 cell lines at 72-and 120-hour exposure were used to assess these effects. Early and late apoptosis was detected in Annexin V-FITC/PI assay revealed a shift in population of cells towards early and mid-apoptosis with ART + PTX than with ART and PTX individually. More effects were observed on LNCaP cell lines at both 72-hour and 120-hour exposure. The results for the Caspase 3/7 activity assay showed shift of viable population in all induced samples compared to control. Morphological changes occurred in both cell lines;this was validated in qualitative assessment when examined under the inverted microscope. These findings indicated that ART + PTX suppressed PCa cell proliferation in a dose- and time-dependent manner.

关 键 词：COMBINATION Prostate Cancer ARTESUNATE PACLITAXEL Anticancer Activities 

分 类 号：R73[医药卫生—肿瘤]