Enzyme electrophoresis method in analysis of active components of haemostasis system  

作　　者：Ludmila Ostapchenko Oleksiy Savchuk Nataliia Burlova-Vasilieva 

机构地区：[1]不详

出　　处：《Advances in Bioscience and Biotechnology》2011年第1期20-26,共7页生命科学与技术进展（英文）

摘　　要：The novel modifications of substrate-containing sodium dodecyl sulfate-polyacrylamide gel electrophoresis that can be used for the detection of proteases and its activators are reported. The protease/activator samples were separated on a protein substrate-SDS-polyacrylamide gel. To detect plasminogen activators fibrinogen and Glu-plasminogen were incorporated into the SDS-PAG followed by 1 h incubation at 37?C in thrombin solution (1 NIH/ml). After electrophoresis the gel was stained according to the standard protocol. To detect fibrin-unspecific plasminogen activators from snake venom incubation in thrombin solution was substituted for 12 h incubation in 50 mM Tris-HCl (pH 7.4). To detect fibrinogen-degrading enzymes fibrinogen-containing gel was used. Activity of protease/activator was visualized in the gel as clear bands against the dark background. These new techniques offer several advantages including determination of the quantity and activity of t-PA and urokinase, however cannot be recommended for precise quantification of activators;the total procedure is quite quick and simple;method is convenient tool for detection of novel protein-protein interactions in haemostasis system;the sensitivity of the method is ≤0.01 IU per track.The novel modifications of substrate-containing sodium dodecyl sulfate-polyacrylamide gel electrophoresis that can be used for the detection of proteases and its activators are reported. The protease/activator samples were separated on a protein substrate-SDS-polyacrylamide gel. To detect plasminogen activators fibrinogen and Glu-plasminogen were incorporated into the SDS-PAG followed by 1 h incubation at 37?C in thrombin solution (1 NIH/ml). After electrophoresis the gel was stained according to the standard protocol. To detect fibrin-unspecific plasminogen activators from snake venom incubation in thrombin solution was substituted for 12 h incubation in 50 mM Tris-HCl (pH 7.4). To detect fibrinogen-degrading enzymes fibrinogen-containing gel was used. Activity of protease/activator was visualized in the gel as clear bands against the dark background. These new techniques offer several advantages including determination of the quantity and activity of t-PA and urokinase, however cannot be recommended for precise quantification of activators;the total procedure is quite quick and simple;method is convenient tool for detection of novel protein-protein interactions in haemostasis system;the sensitivity of the method is ≤0.01 IU per track.

关 键 词：Substrate-Containing ELECTROPHORESIS ENZYME ELECTROPHORESIS HAEMOSTASIS PROTEINS Activity PROTEINS Identification 

分 类 号：R73[医药卫生—肿瘤]