Determination of amino-acidic positions important for <i>Ocimum basilicum</i>geraniol synthase activity  

作　　者：Marc J. C. Fischer Sophie Meyer Patricia Claudel Damien Steyer Marc Bergdoll Philippe Hugueney 

机构地区：[1]Institut de Biologie Moléculaire des Plantes, Université de Strasbourg, Strasbourg, France [2]Unit Mixte Recherche Santé Vigne & Qual Vins, Métab Second Vigne, Institut National de Recherche Agronomique, Université de Strasbourg, Colmar, France

出　　处：《Advances in Bioscience and Biotechnology》2013年第2期242-249,共8页生命科学与技术进展（英文）

摘　　要：Terpenes are one of the largest and most diversified families of natural compounds. Although they have found numerous industrial applications, the molecular basis of their synthesis in plants has, until now, not been fully understood. Plant genomes have been shown to contain dozens of terpene synthase (TPS) genes, however knowledge of their amino-acidic protein sequence in not sufficient to predict which terpene(s) will be produced by a particular enzyme. In order to investigate the structural basis of a TPS specificity, we performed site directed mutations in the geraniol synthase from Ocimum basilicum. The results obtained suggest that a specific region on the catalytic site plays an important role in GPP transformation, either by stabilizing the GPP substrate on the catalytic site, or by enabling its transformation into a monoterpenol via an intermediate carbocation.Terpenes are one of the largest and most diversified families of natural compounds. Although they have found numerous industrial applications, the molecular basis of their synthesis in plants has, until now, not been fully understood. Plant genomes have been shown to contain dozens of terpene synthase (TPS) genes, however knowledge of their amino-acidic protein sequence in not sufficient to predict which terpene(s) will be produced by a particular enzyme. In order to investigate the structural basis of a TPS specificity, we performed site directed mutations in the geraniol synthase from Ocimum basilicum. The results obtained suggest that a specific region on the catalytic site plays an important role in GPP transformation, either by stabilizing the GPP substrate on the catalytic site, or by enabling its transformation into a monoterpenol via an intermediate carbocation.

关 键 词：GERANIOL SYNTHASE HETEROLOGOUS Expression Monoterpenols Point Mutations Yeast 

分 类 号：R73[医药卫生—肿瘤]