Lipase Inhibitor from Fruits of <i>Solanum stramonifolium</i>Jacq.  

作　　者：Watinee Chanmee Chanya Chaicharoenpong Amorn Petsom 

机构地区：[1]Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, Thailand [2]Program of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand [3]Research Centre for Bioorganic Chemistry, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand

出　　处：《Food and Nutrition Sciences》2013年第5期554-558,共5页食品与营养科学（英文）

摘　　要：Obesity is usually considered as an overweight or excess body fat, leading to increased health problems. Obesity is a major risk factor for a number of serious diseases. Decreasing dietary fat absorption, through inhibition of pancreatic lipase activity, has been reported to be one of the most effective ways for managing obesity. The present study was aimed at investigating lipase inhibitors from edible plants. A lipase inhibitor was isolated from n-hexane and ethyl acetate extracts of the ripe fruits of Solanum stramonifolium Jacq. by column chromatography and identified by spectral analysis. Its structure was elucidated as (22R)-3β-benzoyloxy-22-hydroxy-4α-methyl-5α-stigmast-7-en-6-one or carpesterol (1). Carpesterol exhibited moderate lipase inhibition activity with IC50 value of 56.0 μg/mL while orlistat, a well- know pancreatic lipase inhibitor, had IC50 value of 3.5 ng/mL. Moreover, the kinetic properties of carpesterol on pancreatic lipase were evaluated. Carpesterol is a competitive inhibitor and exhibited antagonistic interaction when combined with orlistat on lipase inhibition activity.Obesity is usually considered as an overweight or excess body fat, leading to increased health problems. Obesity is a major risk factor for a number of serious diseases. Decreasing dietary fat absorption, through inhibition of pancreatic lipase activity, has been reported to be one of the most effective ways for managing obesity. The present study was aimed at investigating lipase inhibitors from edible plants. A lipase inhibitor was isolated from n-hexane and ethyl acetate extracts of the ripe fruits of Solanum stramonifolium Jacq. by column chromatography and identified by spectral analysis. Its structure was elucidated as (22R)-3β-benzoyloxy-22-hydroxy-4α-methyl-5α-stigmast-7-en-6-one or carpesterol (1). Carpesterol exhibited moderate lipase inhibition activity with IC50 value of 56.0 μg/mL while orlistat, a well- know pancreatic lipase inhibitor, had IC50 value of 3.5 ng/mL. Moreover, the kinetic properties of carpesterol on pancreatic lipase were evaluated. Carpesterol is a competitive inhibitor and exhibited antagonistic interaction when combined with orlistat on lipase inhibition activity.

关 键 词：SOLANUM stramonifolium Jacq. LIPASE INHIBITOR Carpesterol ORLISTAT Obesity 

分 类 号：R73[医药卫生—肿瘤]