Probing the Cell Cycle with Flow Cytometry  

作　　者：George D. Wilson 

机构地区：[1]Department of Radiation Oncology, Beaumont Health System, Royal Oak, MI, USA

出　　处：《Journal of Biomedical Science and Engineering》2014年第9期698-711,共14页生物医学工程（英文）

摘　　要：Flow cytometry is a versatile technique to study different aspects of the cell cycle from subpopulations of cells to detailed cell kinetic information. In this paper a basic review of cell kinetic parameters is presented followed by detailed descriptions of the different flow cytometric methodologies that can be used to extract pertinent information for a particular study. The methodologies range from simple DNA profile analysis, the use of bromodeoxyuridine to cell cycle-associated proteins such as the cyclins.Flow cytometry is a versatile technique to study different aspects of the cell cycle from subpopulations of cells to detailed cell kinetic information. In this paper a basic review of cell kinetic parameters is presented followed by detailed descriptions of the different flow cytometric methodologies that can be used to extract pertinent information for a particular study. The methodologies range from simple DNA profile analysis, the use of bromodeoxyuridine to cell cycle-associated proteins such as the cyclins.

关 键 词：Flow CYTOMETRY Cell Cycle DNA Profile BROMODEOXYURIDINE CYCLINS 

分 类 号：R73[医药卫生—肿瘤]