DNA Gene Expression to Study Immunologic Mechanisms for the Long-Term Cure of Malaria in Babies and Children in South-Western Nigeria  

作　　者：Jerry T. Thornthwaite Akanni E. Olufemi Ayankunle A. Ademola O. A. T. Alli 

机构地区：[1]Cancer Research Institute of West Tennessee, Henderson, USA [2]Medical Laboratory Science Department, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Osun State, Nigeria [3]Pharmacology & Therapeutics Department, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Osun State, Nigeria

出　　处：《Advances in Biological Chemistry》2019年第2期68-87,共20页生物化学进展（英文）

摘　　要：Malaria infection has been a very serious public health problem in Nigeria and most parts of Africa. Development of antimalarial treatments capable of providing a permanent cure for malaria has been a herculean task for drug researchers. A trial of a novel, proprietary blend formulation (TriantimalTM) in gel caps for children (n = 112) and water-soluble, 18.51 nm diameter, NutraNanoSphereTM encapsulated TriantimalTM drops for babies (19.9 Months ± 8.7 SD, n = 15) was conducted in Osogbo, Nigeria. The enrolled subjects were screened for malaria, treated with TriantimalTM for 16 consecutive days and sera collected on days 0, 5, 10, 16, 30, 60, and 730. Also, 31 of the children donated buffy coat samples for the gene expression studies when sera were collected. The children showed 90.2% parasite-free at 60 days and 85.1% at 730 days. The babies revealed 93.3% parasite-free at 60 days. These data show for the first time a real possibility for a cure of malaria in Nigeria. The one-time, low dose, extended treatment and synergism of the natural components minimize the ability of the parasites to develop resistance, while boosting the immune system. Indeed, the DNA amplification data showed that all aspects of the humoral, innate, and innate defensin immunity are involved in the long-term immunity against P. falciparum in which may be termed a type of “in vivo immunization”.Malaria infection has been a very serious public health problem in Nigeria and most parts of Africa. Development of antimalarial treatments capable of providing a permanent cure for malaria has been a herculean task for drug researchers. A trial of a novel, proprietary blend formulation (TriantimalTM) in gel caps for children (n = 112) and water-soluble, 18.51 nm diameter, NutraNanoSphereTM encapsulated TriantimalTM drops for babies (19.9 Months ± 8.7 SD, n = 15) was conducted in Osogbo, Nigeria. The enrolled subjects were screened for malaria, treated with TriantimalTM for 16 consecutive days and sera collected on days 0, 5, 10, 16, 30, 60, and 730. Also, 31 of the children donated buffy coat samples for the gene expression studies when sera were collected. The children showed 90.2% parasite-free at 60 days and 85.1% at 730 days. The babies revealed 93.3% parasite-free at 60 days. These data show for the first time a real possibility for a cure of malaria in Nigeria. The one-time, low dose, extended treatment and synergism of the natural components minimize the ability of the parasites to develop resistance, while boosting the immune system. Indeed, the DNA amplification data showed that all aspects of the humoral, innate, and innate defensin immunity are involved in the long-term immunity against P. falciparum in which may be termed a type of “in vivo immunization”.

关 键 词：TriantimalTM NutraNanoSpheresTM IMMUNITY qPCR Gene Expression PLASMODIUM FALCIPARUM 

分 类 号：R73[医药卫生—肿瘤]