Analysis of apigenin in <i>Blumea balsamifera</i>Linn DC. and its inhibitory activity against aldose reductase in rat lens  

作　　者：Dong Gu Lee So-Youn Mok Changsun Choi Eun Ju Cho Hyun Young Kim Sanghyun Lee 

机构地区：[1]Department of Food and Nutrition, Chung-Ang University, Anseong, South Korea [2]Department of Food Science and Nutrition, Pusan National University, Busan, South Korea [3]Department of Food Science, Gyeongnam National University of Science and Technology, Jinju, South Korea [4]Department of Integrative Plant Science, Chung-Ang University, Anseong, South Korea

出　　处：《Journal of Agricultural Chemistry and Environment》2012年第1期28-33,共6页农业化学和环境（英文）

摘　　要：To investigate the therapeutic potentials of na- tural sources, stepwise polarity fractions of Blumea balsamifera were tested for their ability to inhibit aldose reductase (AR) activity in rat lenses. Of these, the ethyl acetate (EtOAc) fraction exhibited a unique AR inhibitory activity (IC50 value, 0.11 μg/mL). Apigenin was identified from the active EtOAc fraction and exhibited high AR inhibitory activity (IC50 value, 4.03 μM). The content of apigenin was measured in B. balsamifera (0.47 mg/g) by HPLC/UV analysis. Our result suggests that B. balsamifera could be a useful natural source for the development of a novel AR inhibitory agent against diabetic complications.To investigate the therapeutic potentials of na- tural sources, stepwise polarity fractions of Blumea balsamifera were tested for their ability to inhibit aldose reductase (AR) activity in rat lenses. Of these, the ethyl acetate (EtOAc) fraction exhibited a unique AR inhibitory activity (IC50 value, 0.11 μg/mL). Apigenin was identified from the active EtOAc fraction and exhibited high AR inhibitory activity (IC50 value, 4.03 μM). The content of apigenin was measured in B. balsamifera (0.47 mg/g) by HPLC/UV analysis. Our result suggests that B. balsamifera could be a useful natural source for the development of a novel AR inhibitory agent against diabetic complications.

关 键 词：Blumea balsamifera APIGENIN ALDOSE REDUCTASE Inhibition HPLC 

分 类 号：R28[医药卫生—中药学]