国家自然科学基金(30400313)

作品数:5被引量:18H指数:2
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相关作者:雷明刚邓昌彦熊远著郑嵘姜勋平更多>>
相关机构:华中农业大学更多>>
相关期刊:《Acta Biochimica et Biophysica Sinica》《农业生物技术学报》《自然科学进展》更多>>
相关主题:MEISHANHETEROSISPORCINE背最长肌梅山猪更多>>
相关领域:生物学农业科学更多>>
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Cloning and Identification of Porcine HSPC117 Gene Differentially Expressed in F_(1)Crossbreds and Their Parents被引量:2
《Agricultural Sciences in China》2007年第5期613-619,共7页XIE Hong-tao LEI Ming-gang XIONG Yuan-zhu DENG Chang-yan JIANG Si-wen LI Feng-e ZUO Bo XU De-quan 
financially supported by National Basic Research Program of China(973 Program,2006CB102102);National Natural Science Foundation of China(30400313).
To investigate the molecular basis of porcine heterosis, suppression subtractive hybridization (SSH) was performed to detect the differences in gene expression between porcine longissimus dorsi of Meishan X Large Wh...
关键词:pig HSPCll7 gene longissimus dorsi suppression subtractive hybridization (SSH) rapid amplification of cDNA ends (RACE) 
Molecular Cloning and Functional Analysis of MRLC2 Differential Expressed in Meishan×Yorkshire F1 Crossbreeds and Their Parents,Meishan Pigs被引量:2
《Acta Biochimica et Biophysica Sinica》2006年第11期788-794,共7页Hong-Tao XIE Ming-Gang LEI Yuan-Zhu XIONG Chang-Yah DENG Si-Wen JIANG Bo ZUO Feng-E LI De-Quan XU Tao WANG 
This work was supported by the grants from the Major State Basic Research Development Program of China (No.200016105);the National Natural Science Foundation of China (30400313)
In order to detect the molecular basis of heterosis in pigs, suppression subtractive hybridization was carried out to investigate the difference in gene expression in the Longissimus dorsi muscle tissues between Meish...
关键词:MRLC2 gene pig HETEROSIS Longissimus dorsi suppression subtractive hybridization rapid amplification of cDNA end 
Identification of a Differentially Expressed Gene PPP1CB between Porcine Longissimus dorsi of Meishan and Large White×Meishan Hybrids被引量:9
《Acta Biochimica et Biophysica Sinica》2006年第7期450-456,共7页Tao HUANG Yuan-Zhu XIONG Ming-Gang LEI De-Quan XU Chang-Yan DENG 
This work was supported by the grants from the National Natural Science Foundation of China(No.30400313);the National High Technology Research;Development Program of China;the Major State Basic Research Development Program of China
To study the molecular basis of heterosis, suppression subtractive hybridization was used to investigate the differences in gene expression between porcine Longissimus dorsi of F1 hybrids Large WhitexMeishan and their...
关键词:differential gene expression PIG HETEROSIS PPP1CB suppression subtractive hybridization 
大梅与梅山猪背最长肌正反消减文库的构建及序列分析被引量:4
《农业生物技术学报》2006年第4期456-461,共6页黄涛 熊远著 徐德全 邓昌彦 雷明刚 蒋思文 郑嵘 姜勋平 李凤娥 李家连 左波 
国家高技术研究与发展计划(863)项目(No.2004AA222170);国家自然科学基金(No.30400313);国家重大基础研究发展规划(973)项目(G2000016105)资助。
为从基因差异表达的角度研究杂种优势的分子机理,构建了差减效率均为210倍的大梅与梅山猪背最长肌正反消减文库。在大梅(被消减)-梅山库中挑选了610个有效克隆,经斑点杂交筛选出48个阳性克隆,测序后经Blastn网上比对揭示了37个基因,其...
关键词:抑制消减杂交 杂种优势 消减cDNA文库 肌肉组织 表达序列标签 基因 
一个在梅山猪×大白猪杂交组合的背最长肌中差异表达的新基因的分离、cDNA序列分析及组织表达谱被引量:1
《自然科学进展》2005年第9期1129-1133,共5页刘永刚 雷明刚 熊远著 邓昌彦 
国家自然科学基金(批准号:30400313);国家"九七三"计划(批准号:G200016105)资助项目
为了揭示猪杂种优势的分子机理,用mRNA差异显示技术从梅山猪×大白猪杂交组合的背最长肌中分离到一个差异表达的基因,并用半定量RT—PCR进行鉴定,随后通过cDNA末端快速扩增法(RACE)得到该基因的cDNA全长.经与GenBank进行Blast比较,这个...
关键词: 杂种优势 MRNA差异显示 背最长肌 PRA1family protein 3 CDNA序列分析 组织表达谱 新基因 
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