国家自然科学基金(s30170228)

作品数:2被引量:0H指数:0
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相关期刊:《Chinese Journal of Chemistry》《Science China Chemistry》更多>>
相关主题:ZNF191INTERACTIONFUSION_PROTEIND-GALTHREE更多>>
相关领域:生物学理学更多>>
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Expression and Purification of ZNF191(243-368) in Three Expression Systems
《Chinese Journal of Chemistry》2007年第11期1739-1742,共4页赵东欣 滕欣辰 丁志春 黄仲贤 
Project supported by the National Natural Science Foundation of China (Nos. 30170228, 39990600).Acknowledgements We thank Prof. Long Yu (Fudan University) for the ZNF191 gene.
ZNF191 (243-368), a new human zinc finger protein, probably relates to some hereditary diseases and cancers, To obtain adequate amount of ZNF191(243-368) for the study of its property, structure and function, thre...
关键词:protein expression affinity chromatography fusion protein isopropylthio-β-D-galactoside 
Interaction of zinc finger ZNF191(243—368) and its I323W and R327W mutants with oligonucleotides by florescence spectrum
《Science China Chemistry》2003年第1期64-74,共11页周晶 刘玉奇 汤李明 滕昕辰 唐宁 黄仲贤 
This work was supported by the National Natural Science Foundation of China (Grant Nos. 30170228 and 39990600).
Two mutants of the zinc finger protein, ZNF191(243—368) I323W and R327W, are suc-cessfully obtained by site-directed mutagenesis. The fluorescence spectrum is used to study the interaction between these two mutants a...
关键词:zinc finger protein ZNF191(243-368)  SITE-DIRECTED mutagenesis  fluorescence spectrum  PROTEIN-DNA interaction. 
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