国家自然科学基金(s30400081)

作品数：4被引量：2H指数：1
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相关期刊：《Chemical Research in Chinese Universities》更多>>
相关主题：K1HYPERHYPERTHERMOPHILICEFFECTSACETATE更多>>
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Effect of Calcium Cation on Thermophilic Acylamino Acid-releasing Enzyme Ape1547 from Aeropyrum pernix K1
《Chemical Research in Chinese Universities》2009年第4期513-517,共5页ZHOU Jun XIE Gui-qiu CAO Shu-gui GAO Ren-jun 
Supported by the National Natural Science Foundation of China(Nos.30400081 and 20772046)
The gene of enzyme（Ape1547） was cloned from hyperthermophilic archaeon Aeropyrum pernix K1 and expressed in Escherichia coil.The effect of calcium cation on the properties of Ape1547 was studied.Ape1547 exhibits bot...
关键词：Acylamino acid-releasing enzyme Aeropyrum pernix K1 Conformational change Calcium cation 
Study on a Specific Site Tyr^(444) on a Hyperthermophilic Enzyme APE1547
《Chemical Research in Chinese Universities》2009年第3期353-356,共4页RAO Lang BI Yun-feng XIE Gui-qiu ZHANG Fei WANG Yan CAO Shu-gui GAO Ren-jun 
Supported by the National Natural Science Foundation of China(Nos.30400081 and 20432010)
Hyperthermophilic enzyme APE1547 is an extremely thermostable recombinant protein from thermophilic archaeon Aeropyrumpernix K1. The Tyr444 located in the catalytic domain adjacent to the catalytic amino acid Ser445 a...
关键词：Hyperthermophilic enzyme APE 1547 Site-directed mutagenesis Thermostability ACTIVITY 
High-throughput Screening of the Enantioselectivity of Hyperthermophilic Mutant Esterases from Archaeon Aeropyrum pernix K1 for Resolution of (R,S)-2-Octanol Acetate被引量：1
《Chemical Research in Chinese Universities》2007年第3期319-324,共6页ZHANG Gui-rong GAO Ren-jun ZHANG Ai-jun RAO Lang CAO Shu-gui 
Supported by the National Natural Science Foundation of China(Nos30400081, 30570405 and 20672045);the Key Tech-nology Research and Development Program of China(No2004BA713D03-04)
To identify the desired hypertherrnophilic variants within a mutant esterase library for the resolution of （R, S）-2- octanol acetate, a simple, reliable, and versatile method was developed in this study. We built a ...
关键词：High-throughput screening ENANTIOSELECTIVITY Hyperthermophilic esterase Directed evolution 
Denaturing Effects of Urea and Guanidine Hydrochloride on Hyperthermophilic Esterase from Aeropyrum pernix K1被引量：2
《Chemical Research in Chinese Universities》2006年第2期168-172,共5页GAO Ren-jun XIE Gui-qiu ZHOU Jun FENG Yan CAO Shu-gui 
SupportedbytheNationalNaturalScienceFoundationofChina(Nos.30400081,20272017and30570405)andtheKeyTechnologiesResearchandDevelopmentProgrammer(No.2001BA708B0306).
The changes in the activity and the conformation of the hyperthermophilic esterase derived from aerobic thermophilic Aeropyrum pernix K1 （ APE1547 ） were studied during denaturation by guanidine hydrochlofide （ Gdn...
关键词：STABILITY Hyperthermophilic esterase UREA Guanidine hydrochloride 
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