supported by the National Natural Science Foundation of China (grant No. 31170158 and 31000090);the '100 Talents Project' of Chinese Academy of Sciences, China (grant No. Y002171YC1)
We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secre...