This work was partially supported by the National Natural Science Foundation of China(Grant No.61775241);the Hunan Science Fund for Distinguished Young Scholar(2020JJ2059);Youth Innovation Team(Grant No.2019012)of CSU,Hunan province key research and development project(Grant No.2019GK2233,Grant 2020SK2053);Hunan Province Graduate Research and Innovation Project(Grant No.CX20190177);the Science and Technology Innovation Basic Research Project of Shenzhen(Grant No.JCYJ20180307151237242);Also,YPL acknowledges the support by the Project of State Key Laboratory of High-Performance Complex Manufacturing,Central South University(Grant No.ZZYJKT2020-12).Besides,we acknowledge the art work from Servier Medical Art.Y.Z.O and Y.P.L contributed equally to this work.
Fluorescence lifetime imaging microscopy(FLIM)has been rapidly developed over the past 30 years and widely applied in biomedical engineering.Recent progress in fluorophore-dyed probe design has widened the application...