We thank Jian-Min Zhou (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for critical comments on the manuscript, Weicai Yang (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for providing binary vector pWM101 and assistance in isolating mitochondria and chloroplasts, Qijun Chen (China Agricultural University) for providing CRISPR/Cas9 binary vector pHEE2A-TRI, and Nottingham Arabidopsis Stock Centre (NASC) for providing som787-2 (N877710), som328-2 (N362639), dit2.1-1 (N663245), dit2.1-2 (N669411), dit2.2-1 (N661169), dit2.2-2 (N655976), and Isdl (N542687) seeds. This work was supported by the National Natural Science Foundation of China (31521001, 31661143025), and the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB13030300).
Programmed cell death (PCD) is a fundamental biological process. Deficiency in MOSAIC DEATH 1 (MOD1), a plastid-localized enoyl- ACP reductase, leads to the accumulation of reactive oxygen species (ROS) and PCD,...
We reconstituted bilayer nuclear membranes, multilayer membranes, and organelles from mixtures ofXenopus laevis egg extracts and demembranatedXenopus sperm nuclei. Varying proportions of the cytosolic and vesicular fr...