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Preparation of biomimetic gene hydrogel via polymerase chain reaction for cell-free protein expression被引量：1
《Science China Chemistry》2020年第1期99-106,共8页Feng Li Wenting Yu Xue Zhang Xiaocui Guo Xihan Xu Xiaolei Sun Dayong Yang 
supported by the National Natural Science Foundation of China(21621004,21575101,21622404)
Deoxyribonucleic acid(DNA)hydrogels,a three-dimensional(3 D)network made from DNA chains,have attracted great attention because of its molecular programmability,excellent biocompatibility and wide biomedical applicati...
关键词：HYDROGEL DNA polymerase chain reaction free radical polymerization biomimetic materials 
Biological activity assays of cell-free reassembled nuclei——Injecting cell-free reassembled nuclei into unfertilized eggs can induce the eggs to cleave and reconstitute asters,and the injected nuclei undergo cell cycle changes
《Science China Chemistry》1995年第9期1075-1083,共9页张传茂 曲健 梁金华 翟中和 
Nucleus may reassemble spontaneously in cell-free mixture of HeLa metaphase chromosomes,Xenopus egg extracts and ATP-regenerating system,and the nucleus shows some biological activities.It isfound that,after being inj...
关键词：NUCLEAR reassembly in CELL-FREE system NUCLEAR transplantation BIOLOGICAL activity of nucleus aster reassembly. 
Chromatin and Nucleosome Organizations and DNA Replication of Nucleus Reassembled in vitro Using Purified Exogenous DNA and Xenopus Egg Extracts
《Science China Chemistry》1994年第6期677-690,共14页张传茂 张博 翟中和 
Project supported by the National Natural Science Foundation of China.
It has been demonstrated in the last ten years that the nuclear reassembly may occur in the cell-free systems from frog egg extracts added with exogenous naked DNA. However, there remains an open question : is the cel...
关键词：CELL-FREE system nuclear reassembly CHROMATIN RECONSTITUTION NUCLEOSOME DNA replication. 
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