MUTANTS

作品数：255被引量：866H指数：14
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A novel transcription factor FnMYB4 regulates pigments metabolism of yellow leaf mutants in Fragaria nilgerrensis
《Horticultural Plant Journal》2024年第5期1134-1150,共17页Shu Jiang Yi Ji Jingyu Yue Mingqian Wang Yumeifeng Jia Li Xue Jiajun Lei 
the National Natural Science Foundation of China(Grant No.32372652);the Liaoning Provincial Science and Technology Project of‘Jiebangguashuai’(Grant No.2022JH1/10400016);the Shenyang Academician and Expert Workstation Project(Grant No.2022-15).
The strawberry species Fragaria nilgerrensis Schlechtendal ex J.Gay,renowned for its distinctive white,fragrant peach-like fruits and strong disease resistance,is an exceptional research material.In a previous study,a...
关键词：Fragaria nilgerrensis Mutant Leaf yellowing RNA-seq Flavonoid MYB 
CRISPR/Cas9-mediated NlInR2 mutants:Analyses of residual mRNA and truncated proteins
《Journal of Integrative Agriculture》2024年第6期2006-2017,共12页Jun Lü Jingxiang Chen Yutao Hu Lin Chen Shihui Li Yibing Zhang Wenqing Zhang 
the National Natural Science Foundation of China(31730073).
CRISPR/Cas9 technology is a powerful genome manipulation tool in insects.However,little is known about whether mRNA and protein of a target gene are completely cleared in homozygous mutants.This study generated homozy...
关键词：CRISPR/Cas9 Nilaparvata lugens residual mRNA skipping exon truncated protein 
Development of a single transcript CRISPR/Cas9 toolkit for efficient genome editing in autotetraploid alfalfa
《The Crop Journal》2024年第3期788-795,共8页Haixia Zhao Siyi Zhao Yingping Cao Xiping Jiang Lijuan Zhao Zhimeng Li Mengqi Wang Ruijuan Yang Chuanen Zhou Zhaoming Wang Feng Yuan Dongmei Ma Hao Lin Wenwen Liu Chunxiang Fu 
supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA26030301);Hohhot Key R&D Project(2023-JBGSS-1),the National Natural Science Foundation of China(U23A200206,32071864,32325035);the Taishan Scholar Program of Shandong(to Chunxiang Fu);the Shandong Provincial Natural Science Foundation(ZR202210270038)。
Alfalfa(Medicago sativa.L.)is a globally significant autotetraploid legume forage crop.However,despite its importance,establishing efficient gene editing systems for cultivated alfalfa remains a formidable challenge.I...
关键词：ALFALFA Gene editing CRISPR_2.0 toolkit Hairy root system Tetra-allelic homozygous mutants 
A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection of Cas9-free progeny in Arabidopsis被引量：1
《aBIOTECH》2024年第2期140-150,共11页Feng-Zhu Wang Ying Bao Zhenxiang Li Xiangyu Xiong Jian-Feng Li 
supported by the National Key Research and Development Program of China(grant 2019YFA0906202);J.-F.L.,the National Natural Science Foundation of China(grants 31900305 and 32370294);the Natural Science Foundation of Guangdong Province(grant 2020A1515010465)to F.-Z.W.
The CRISPR/Cas9 technology revolutionizes targeted gene knockout in diverse organisms including plants.However,screening edited alleles,particularly those with multiplex editing,from herbicide-or antibiotic-resistant ...
关键词：CRISPR Genome editing D-amino acid oxidase Cas9-free Selection marker 
Enhancing the endo-activity of the thermophilic chitinase to yield chitooligosaccharides with high degrees of polymerization
《Bioresources and Bioprocessing》2024年第1期407-419,共13页Feifei Guan Xiaoqian Tian Ruohan Zhang Yan Zhang Ningfeng Wu Jilu Sun Honglian Zhang Tao Tu Huiying Luo Bin Yao Jian Tian Huoqing Huang 
supported by the National Key R&D Program of China[Grant No.2021YFC2103002];the National Natural Science Foundation of China[Grant No.32202720];The Agricultural Science and Technology Innovation Program(CAAS-ZDRW202304);the China Agriculture Research System of MOF and MARA(CARS-41).
Thermophilic endo-chitinases are essential for production of highly polymerized chitooligosaccharides,which are advantageous for plant immunity,animal nutrition and health.However,thermophilic endo-chitinases are scar...
关键词：CHITIN CHITINASE Endo-activity MUTANTS Functional oligosaccharides 
Development of Chlorella pyrenoidosa EMS mutants with enhanced biomass and lipid content for biofuel production
《Systems Microbiology and Biomanufacturing》2023年第4期693-701,共9页M.Vidya Vani P.Osman Basha N.Rajesh K.Riazunnisa 
Biodiesel production from microalgae depends on algal biomass production through autotrophic cultivation,and it has a lot of potential as a feasible alternative source of energy.The lethal and mutagenic effects of eth...
关键词：Chlorella pyrenoidosa Random mutagenesis Ethyl methanesulphonate(EMS) Chlorophyll mutants 
Improvement of lycopene biosynthesis in waaC and waaF mutants of Escherichia coli by integrant expression of crtEBI gene and deletion of aceE and gdhA
《Systems Microbiology and Biomanufacturing》2023年第4期739-749,共11页Xiaoqing Hu Mao Cui Xiaoyuan Wang 
supported by the National Key research and Development Program of China(2018YFA0900302);the National Science Foundation of China(31970045);the National First-class Discipline Program of Light Industry Technology and Engineering(LITE2018-12);the Program of Introducing Talents of Discipline to Universities(111-2-06);Top-notch Academic Programs Project of Jiangsu Higher Education Institutions.
As a bright red carotenoid pigment found in tomatoes and other red fruits,lycopene was efficiently produced by Escherichia coli transformed with the genes involving in lycopene biosynthesis.Our previous work showed th...
关键词：LYCOPENE Escherichia coli waaC waaF 
A multi-omics approach identifies bHLH71-like as a positive regulator of yellowing leaf pepper mutants exposed to high-intensity light被引量：2
《Horticulture Research》2023年第7期40-52,共13页Zhoubin Liu Lianzhen Mao Bozhi Yang Qingzhi Cui Yunhua Dai Xueqiao Li Yisong Chen Xiongze Dai Xuexiao Zou Lijun Ou Sha Yang 
This research was funded by the Special Project of Biological Seed Industry and Fine and Deep Processing of Agricultural Products(grant 202202AE090031);the Project of Education Department of Hunan Province(grant 22B0229);the Key Research and Development Program of Hainan Province(grant ZD2020060).
Light quality and intensity can have a significant impact on plant health and crop productivity.Chlorophylls and carotenoids are classes of plant pigments that are responsible for harvesting light energy and protectin...
关键词：HARVESTING BHLH yellow 
CRISPR/Cas9-mediated SNAC9 mutants reveal the positive regulation of tomato ripening by SNAC9 and the mechanism of carotenoid metabolism regulation被引量：2
《Horticulture Research》2023年第4期1-12,共12页Yuan Feng Xiaohong Kou Shuai Yuan Caie Wu Xiaoyang Zhao Zhaohui Xue Qingxiu Li Zhengyu Huang Yijie Sun 
supported by the National Natural Science Foundation of China,China[Grant No.32072274 and 31871848].
NAC transcriptional regulators are crucial for tomato ripening.Virus-induced gene silencing(VIGS)of SNAC9(SlNAC19,Gene ID:101248665)affects tomato ripening,and SNAC9 is involved in ethylene and abscisic acid(ABA)metab...
关键词：CRISPR/Cas9 METABOLISM MECHANISM 
Construction of Zebrafish Mutants of CNTF Gene Using CRISPR/Cas9 Genome Editing Technology
《Journal of Biosciences and Medicines》2023年第11期269-276,共8页Lihan Xu Rong He Feihao Shen Keyun Wang Yingjia Wang Linyi Hu Meihua Ye Yan Zhang Yuying Wang 
Background: The prevalence of Parkinson’s disease (PD), a chronic and progressive neurodegenerative disorder, is projected to increase twofold by 2030. Leucine-rich repeat kinase 2 (LRRK2) is the most commonly observ...
关键词：Environment Pollution Ultraviolet Stabilizer Parkinson’s Disease Ciliary Neurotrophic Factor ZEBRAFISH CRISPR/Cas9 
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