Protoplasts isolated from immature cotyledons of cultivated soybean (Glycine max L.) were cultured by Gelrite bead method in ZSP medium with soybean root nodule products (asparagine, glutamine, allantoin, allantoic ac...
Protoplasts were isolated from embryogenic calli derived from immature embryos ofwheat (Triticum aestivum L. cv. Jinan 177). The protoplasts were cultured in NMB mediumsupplemented with 1mg/L 2,4- D and 500mg/l casein...
This work was supported in part by the Monsando Inc., USA.
Embryogenic calli were induced from the mature seeds of the hexaploid semi-winter wheat. The embryogenic cell line was established, and then the friable calli suitable for suspension were induced by adjusting the cont...
Genetic engineering of soybean is limited because of the difficulty of regeneration of plants via in vitro culture. Here we report that a method of obtaining somatic embryos and regenerated plants of soybean has been ...